FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_94.3510000004f93a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_94.3510000004f93a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166307
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA10014560.21694817415983Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA23481.4118467653195597RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA12230.7353869650706224RNA PCR Primer, Index 31 (96% over 31bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA7470.44916930736529426Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA7090.4263199985568858RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA4790.28802155050599193Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA4700.2826098721040004RNA PCR Primer, Index 31 (96% over 31bp)
GTCCACGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3490.20985286247722584RNA PCR Primer, Index 31 (96% over 31bp)
GTCCACGAGGTCGACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA2870.17257241126350664RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC2780.16716073286151514RNA PCR Primer, Index 31 (96% over 31bp)
GAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACT2770.16655943526129388No Hit
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA2760.1659581376610726RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCGTGAAAAAAA2440.1467166144539917RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA1970.11845562724359167No Hit
GTCCACGAGGTCGACATATCGTATGCCGTCTTCTGCTTGAAAAAAA1700.10222059203761717RNA PCR Primer, Index 41 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGC200.001252674540.030
CTCTGGT850.040.012
CTCTGGG600.040.012
GTATGTT304.8424117E-640.034
TGATGAT200.001252674540.015
CTCTGAA600.040.012
CGTCAGG304.8424117E-640.09
GAGGTAG200.001252674540.07
TTTTGCT200.001252674540.031
ACTGCAC200.001252674540.020
CCAATTG304.8424117E-640.028
CAAGACG257.765896E-540.023
AACCGTC304.8424117E-640.06
AAACCAA451.207809E-940.028
ACTAAAA451.207809E-940.023
GTGTCGA257.765896E-540.021
CCAACAT257.765896E-540.022
AACAACT451.207809E-940.015
TCTAGTA200.001252674540.013
TCTAGCT304.8424117E-640.013
Produced by FastQC (version 0.11.2)