Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-AKRU9_l01n01_exp163gap1bff_93.3510000004f92d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2209156 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 164840 | 7.461673145762454 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC | 18240 | 0.8256546844134139 | No Hit |
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA | 9850 | 0.4458716360456211 | No Hit |
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 4226 | 0.19129477501815173 | RNA PCR Primer, Index 41 (96% over 28bp) |
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA | 3183 | 0.14408217436885398 | RNA PCR Primer, Index 41 (96% over 30bp) |
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA | 2917 | 0.13204137688782502 | No Hit |
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG | 2399 | 0.10859350810897916 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTTTGAT | 145 | 0.0 | 40.000004 | 29 |
GAAATGA | 175 | 0.0 | 40.000004 | 24 |
TTGTGGA | 145 | 0.0 | 40.000004 | 15 |
GCGAATT | 145 | 0.0 | 40.000004 | 23 |
CTTTTGT | 175 | 0.0 | 40.000004 | 14 |
CTTTTCG | 145 | 0.0 | 40.000004 | 14 |
CGTTGGC | 245 | 0.0 | 40.000004 | 30 |
GCTATCA | 145 | 0.0 | 40.000004 | 17 |
TTCACCT | 135 | 0.0 | 40.000004 | 15 |
AAGGTTT | 215 | 0.0 | 40.000004 | 26 |
TGCGGTG | 135 | 0.0 | 40.000004 | 29 |
AGGCGAA | 135 | 0.0 | 40.000004 | 22 |
CATTCAA | 135 | 0.0 | 40.000004 | 22 |
TGTGCGG | 185 | 0.0 | 40.000004 | 28 |
GTGTGTT | 145 | 0.0 | 40.000004 | 28 |
AAACGGT | 135 | 0.0 | 40.000004 | 25 |
TACGCAA | 265 | 0.0 | 40.000004 | 21 |
TCATGAG | 270 | 0.0 | 40.000004 | 15 |
GTCGTCG | 245 | 0.0 | 40.000004 | 31 |
AGACAAT | 290 | 0.0 | 40.000004 | 23 |