FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_93.3510000004f92d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_93.3510000004f92d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209156
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1648407.461673145762454Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC182400.8256546844134139No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA98500.4458716360456211No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA42260.19129477501815173RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA31830.14408217436885398RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA29170.13204137688782502No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG23990.10859350810897916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAT1450.040.00000429
GAAATGA1750.040.00000424
TTGTGGA1450.040.00000415
GCGAATT1450.040.00000423
CTTTTGT1750.040.00000414
CTTTTCG1450.040.00000414
CGTTGGC2450.040.00000430
GCTATCA1450.040.00000417
TTCACCT1350.040.00000415
AAGGTTT2150.040.00000426
TGCGGTG1350.040.00000429
AGGCGAA1350.040.00000422
CATTCAA1350.040.00000422
TGTGCGG1850.040.00000428
GTGTGTT1450.040.00000428
AAACGGT1350.040.00000425
TACGCAA2650.040.00000421
TCATGAG2700.040.00000415
GTCGTCG2450.040.00000431
AGACAAT2900.040.00000423