FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_92.3510000004f910.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_92.3510000004f910.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280179
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA12800.45685079895352615No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA7960.2841040905992241Illumina PCR Primer Index 11 (96% over 28bp)
AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGTCCCTAGTTTTGT3850.1374121543727403No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG3300.11778184660520595No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3300.11778184660520595No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA3210.11456961442506397No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3210.11456961442506397No Hit
AAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACAT2860.10207760039117848No Hit
AGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATATGCACA2850.10172068570449605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA1850.040.00000413
CTCTTGT1850.040.00000412
TCTAGAT3700.040.00000413
CGGACAA353.045352E-740.022
TCCGGCT200.001254144240.015
CCTTCGC353.045352E-740.028
ATTTATT200.001254144240.027
CTTAATT200.001254144240.029
ATTTATA257.779551E-540.024
TTGATTT200.001254144240.030
CTTGTGG257.779551E-540.014
TCTGCCT1750.040.013
GTTTGTT257.779551E-540.024
CTTCGTT257.779551E-540.014
GTGTTGC304.854326E-640.030
CTTAAGA650.040.014
TCGCCAG401.9193976E-840.015
ATTTACC304.854326E-640.021
ACAACGC451.2150849E-940.025
ATCCTGT200.001254144240.028
Produced by FastQC (version 0.11.2)