FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_91.3510000004f903.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_91.3510000004f903.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297334
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA89783.019499956278125Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA13320.44798105833843427No Hit
GGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGC6110.20549281279638384No Hit
CAAAAAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAA4630.15571713964766895No Hit
CGTATTTAGCCACATAGAAACCAACAGCCATATAACTGGTAGCTTT4610.15504449541592957No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG3590.12073963959722063No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3490.11737641843852367No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3210.10795939919417222No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA3190.10728675496243281No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA3180.10695043284656312No Hit
ATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGC3070.10325088957199646No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG3050.1025782453402571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA257.7806995E-540.00000422
TGATCAC257.7806995E-540.00000415
CCTTCGC257.7806995E-540.00000428
TTGATTT257.7806995E-540.00000430
ACAACTT257.7806995E-540.00000424
GTTTGTC257.7806995E-540.00000430
GTGTTGT257.7806995E-540.00000423
CTTAAGA507.6397555E-1140.00000414
GTTGAAA257.7806995E-540.00000421
TGTTTCC257.7806995E-540.00000430
GAGGATA257.7806995E-540.00000423
GTGCAAA257.7806995E-540.00000420
AGCGTGG257.7806995E-540.00000419
CGTGGGC257.7806995E-540.00000428
CGTGGAA257.7806995E-540.00000421
CGGAAGT257.7806995E-540.00000425
CAGCGGA257.7806995E-540.00000427
CTGTCTT257.7806995E-540.00000418
TAATAAT257.7806995E-540.00000425
AGCAAAG257.7806995E-540.00000418
Produced by FastQC (version 0.11.2)