FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_90.3510000004f8f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_90.3510000004f8f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311284
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA13280.4266200639930096No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA12400.3983500597525089Illumina PCR Primer Index 11 (96% over 28bp)
TGATGAGACAGGCCGTTTGAATGTTGACGGGATGAACATAATAAGC4540.1458475218771283No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG3900.12528751879312783No Hit
TAAGCAGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCT3810.12239626835944026No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3530.11340126701019006No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA3470.111473766721065No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3460.1111525166728775No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA3460.1111525166728775No Hit
AGAGCTTGATGCGGTTATCCATCTGCTTATGGAAGCCAAGCATTGG3380.10858251628737745No Hit
CTGCAACAACTGAACGGACTGGAAACACTGGTCATAATCATGGTGG3240.10408501561275235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA257.781543E-540.00000422
CTTAATT257.781543E-540.00000429
TTCTATG257.781543E-540.00000415
AGCGTTT257.781543E-540.00000416
ACAACAA257.781543E-540.00000422
AGACTTT257.781543E-540.00000416
AAACTGA257.781543E-540.00000425
GAAATCG507.6397555E-1140.00000424
GAAATCA257.781543E-540.00000424
TAATAGG507.6397555E-1140.00000415
CAGAAAT257.781543E-540.00000422
CAGCGCC257.781543E-540.00000427
TGATAAA257.781543E-540.00000427
ACAAATC257.781543E-540.00000429
GCAAGTC257.781543E-540.00000424
GTACCAA257.781543E-540.00000419
TCGCAGG257.781543E-540.00000423
GATGTTG257.781543E-540.00000419
GTATTTG257.781543E-540.00000425
GGTGTTG257.781543E-540.00000422
Produced by FastQC (version 0.11.2)