FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_9.3510000004f3ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_9.3510000004f3ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425
Sequences flagged as poor quality0
Sequence length46
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTAT34014.02061855670103No Hit
TAAGAGCCTCGATACGCTCAAAGTCAAAATAATCAGCGTGACATTC32513.402061855670103No Hit
TCCGAGATTATGCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTC30812.701030927835053No Hit
TTACCTCCAAATGAAGAAATAACATCATGGTAACGCTGCATGAAGT25010.309278350515463No Hit
GCGCCGCCAAAACGTCGGCTACAGTAACTTTTCCCAGCCTCAATCT1676.88659793814433No Hit
GTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT1656.804123711340206No Hit
GGATACGCCAATCATTTTTATCGAAGCGCGCATAAATTTGAGCAGA1636.721649484536083No Hit
AGTGCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGT1496.144329896907216No Hit
AAATTTAATGTGACCGTTTATCGCAATCTGCCGACCACTCGCGATT1355.567010309278351No Hit
GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTC1184.8659793814432994No Hit
TTCTGCTTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCA813.34020618556701No Hit
ATAACGCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTT783.216494845360825No Hit
ACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATA50.2061855670103093No Hit
ATCCATCTGCTTATGGAAGCCAAGCATTGGGGATTGAGAAAGAGTA40.16494845360824742No Hit
ATTAAAGAGCTTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTAT40.16494845360824742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAC650.040.022
TTGTCTC650.040.015
TTTGAGC255.709648E-540.037
AGCCTCG303.1383915E-640.05
GAGGTGA650.040.035
GGTGATT650.040.037
GTTGACT303.1383915E-640.010
GGTTGAC303.1383915E-640.09
CCAATCA255.709648E-540.08
GCCTCGA303.1383915E-640.06
CTGAGGT303.1383915E-640.05
ACGCTCA303.1383915E-640.014
ACGCCAA255.709648E-540.05
CGATACG303.1383915E-640.010
AAATTTG255.709648E-540.034
TGACTTA303.1383915E-640.012
AGCCACT650.040.023
TCGATAC303.1383915E-640.09
CCAGCCA650.040.021
AATCATT255.709648E-540.010
Produced by FastQC (version 0.11.2)