FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_89.3510000004f8e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_89.3510000004f8e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437496
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA43660.9979519812752574Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA20940.47863294750123425No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA5380.12297255289191215No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA5320.12160111178159343No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA5030.11497247974838627No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG4880.11154387697258947No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA4860.11108672993581656No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG4570.10445809790260939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA401.9235813E-840.00000422
CCTTCGC200.001254916240.00000428
ATTTATT401.9235813E-840.00000427
ATTTATA200.001254916240.00000424
GTGTTTA200.001254916240.00000428
ACAACTT200.001254916240.00000424
GTTTGTT401.9235813E-840.00000424
GTTGAGA200.001254916240.00000424
GTGTTGC401.9235813E-840.00000430
ATCCTGT200.001254916240.00000428
AAACTTT200.001254916240.00000424
CTTCGGT200.001254916240.00000414
GTTGAAA200.001254916240.00000421
TGTTTCC401.9235813E-840.00000430
GAGGATG401.9235813E-840.00000423
AGACTTA200.001254916240.00000427
AGGTATT200.001254916240.00000427
AGCAATT401.9235813E-840.00000423
ATGGGCG200.001254916240.00000426
CTAGAAT401.9235813E-840.00000414