FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_88.3510000004f8db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_88.3510000004f8db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5290
Sequences flagged as poor quality0
Sequence length46
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAACATAAATC54210.245746691871457No Hit
AACATAAATCACCTCACTTAAGTGGCTGGAGACAAATAATCTCTTT4889.224952741020793No Hit
TCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGG4488.468809073724008No Hit
GAACGGAAAACATCCTTCATAGAAATTTCACGCGGCGGCAAGTTGC4107.750472589792061No Hit
ACAGAAACAACCTGATTAGCGGCGTTGACAGATGTATCCATCTGAA3576.748582230623819No Hit
TAAACGTGACGATGAGGGACATAAAAAGTAAAAATGTCTACAGTAG3476.559546313799622No Hit
AGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGAAGTGCCAG3155.954631379962192No Hit
GAAACAAAACTAGGGACGGCCTCATCAGGGTTAGGAACATTAGAGC3065.784499054820416No Hit
TCCTTCATGAACTTAATCCACTGTTCACCATAAACGTGACGATGAG3035.727788279773157No Hit
TTACCAGCATTAACCGTCAAACTATCAAAATATAACGTTGACGATG2975.614366729678639No Hit
TTATCGAACTCAACGCCCTGCATACGAAAAGACAGAATCTCTTCCA2745.179584120982987No Hit
GAAGGCGGTTCCTGAATGAATGGGAAGCCTTCAAGAAGGTGATAAG2614.933837429111531No Hit
GAAGCAGCATCAGTGACGACATTAGAAATATCCTTTGCAGTAGCGC2304.3478260869565215No Hit
ATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCT1833.459357277882798No Hit
TGATTATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAAT1512.854442344045369No Hit
GCACGCTCCCAAGCATTAAGCTCAGGAAATGCAGCAGCAAGATAAT1482.7977315689981097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATA304.0062405E-640.00000432
AACGTTG304.0062405E-640.00000434
CGTCAAA304.0062405E-640.00000415
AACCGTC304.0062405E-640.00000412
GTTGACG304.0062405E-640.00000437
TTGCAGT600.040.00000435
AAACTAT304.0062405E-640.00000419
CAGCATT304.0062405E-640.0000045
AAATCAA304.0062405E-640.00000410
CCAAATC304.0062405E-640.0000048
TTACCAG304.0062405E-640.0000041
CCAGCAT304.0062405E-640.0000044
ACGTTGA304.0062405E-640.00000435
TAAGTGG304.0062405E-640.00000419
TAACGTT304.0062405E-640.00000433
AATCAAG304.0062405E-640.00000411
GTCAAAC304.0062405E-640.00000416
AAATATA304.0062405E-640.00000428
TATAACG304.0062405E-640.00000431
AGTGGCT304.0062405E-640.00000421