FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_87.3510000004f8ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_87.3510000004f8ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGA47914.93607733083879No Hit
TTACTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAAT3039.448082319925163No Hit
CAGATTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCATT2377.390084190832553No Hit
CTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGCGCAGCTCG1484.614904895541004No Hit
GGCCTCCAGCAATCTTGAACACTCATCCTTAATACCTTTCTTTTTG1163.617087620829436No Hit
ATAGTCAGGTTAAATTTAATGTGACCGTTTATCGCAATCTGCCGAC1123.4923604614904895No Hit
TTAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCCGACTGT1083.367633302151544No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA832.5880885562831306Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA90.2806361085126286No Hit
GTCCACGAGGTCTCTAGACTCTCCTTAGACTGTGTCGTACGCTGCA50.1559089491736826No Hit
GTCCACGAGGTCTCTGCGCTCGATATAACTTCATCCGTACGCTGCA40.12472715933894606No Hit
GTCCACGAGGTCTCTCCCGCATTATTATACACGAGCGTACGCTGCA40.12472715933894606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGA303.517789E-640.026
CTCTGCT200.001077414740.012
TGTGAGC303.517789E-640.013
CAAGATG256.188006E-540.039
GAGGTCT1550.040.07
GAGGTCG200.001077414740.07
ACTAATA351.9777872E-740.030
TTGTCAT303.517789E-640.06
GTATGCC200.001077414740.021
CATTGTG303.517789E-640.010
TTCATCC303.517789E-640.023
CTGAGTC351.9777872E-740.08
GTCATTG303.517789E-640.08
ATCTCGT200.001077414740.016
TCAAGAT256.188006E-540.038
AGCTTAA256.188006E-540.031
TCGACAT200.001077414740.011
TGCCGTC200.001077414740.024
ACCACTA351.9777872E-740.027
TTACTAT303.517789E-640.01
Produced by FastQC (version 0.11.2)