FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_86.3510000004f8b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_86.3510000004f8b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258306
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA11420.4421112943563061No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA7590.29383754152052216Illumina PCR Primer Index 11 (96% over 28bp)
CCATCCTTCATGAACTTAATCCACTGTTCACCATAAACGTGACGAT3740.14478951321301092No Hit
AAAAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA3720.14401523774128358No Hit
GATACAAACTCATCACGAACGTCAGAAGCAGCCTTATGGCCGTCAA3460.1339496566088283No Hit
GTCGGGAGAGGAGTGGCATTAACACCATCCTTCATGAACTTAATCC3460.1339496566088283No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3260.12620690189155498No Hit
TCATGGTAACGCTGCATGAAGTAATCACGTTCTTGGTCAGTATGCA3100.12001269811773632No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA3070.11885128491014532No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2850.11033425472114469No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2810.10878570377769003No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA2730.1056886018907807No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG2690.10414005094732603No Hit
AAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA2670.10336577547559869No Hit
ATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGG2620.10143008679628038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT200.001253962340.00000415
CCTTCGC200.001253962340.00000428
ATTTATT200.001253962340.00000427
CTTAATT200.001253962340.00000429
TCTGCCT1600.040.00000413
CTTGTCC200.001253962340.00000414
GTTTGTT401.9183062E-840.00000424
CTTGTAT200.001253962340.00000414
GTGTTGC200.001253962340.00000430
ACAACGC401.9183062E-840.00000425
GTTGAAA304.852851E-640.00000421
CTTCGGG200.001253962340.00000418
GTTTGAT200.001253962340.00000429
TGTTTAG304.852851E-640.00000429
CTTCGAG600.040.00000414
AACGTGC200.001253962340.00000426
CGGAATG200.001253962340.00000428
ACTATTT200.001253962340.0000041
ATGGGAC200.001253962340.00000421
AAGTAAT304.852851E-640.00000419
Produced by FastQC (version 0.11.2)