Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-AKRU9_l01n01_exp163gap1bff_85.3510000004f8a5.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 461820 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 56 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 2190 | 0.47421073145381315 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA | 2091 | 0.45277380797713396 | No Hit |
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA | 565 | 0.12234203802347235 | No Hit |
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA | 562 | 0.12169243428175479 | No Hit |
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA | 549 | 0.11887748473431206 | No Hit |
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG | 526 | 0.11389718938114417 | No Hit |
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA | 512 | 0.11086570525312893 | No Hit |
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG | 498 | 0.10783422112511368 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCCGGAT | 25 | 7.787405E-5 | 40.0 | 15 |
TCCGGAA | 20 | 0.001254989 | 40.0 | 15 |
TCGCCTA | 30 | 4.8611837E-6 | 40.0 | 15 |
ATTTATT | 25 | 7.787405E-5 | 40.0 | 27 |
ATTTATA | 55 | 5.456968E-12 | 40.0 | 24 |
ACAACTA | 20 | 0.001254989 | 40.0 | 24 |
GTTTGTT | 60 | 0.0 | 40.0 | 24 |
GTTTGTC | 20 | 0.001254989 | 40.0 | 30 |
ATTTAGG | 35 | 3.051082E-7 | 40.0 | 18 |
GTTGAGG | 20 | 0.001254989 | 40.0 | 25 |
GTTGAGA | 30 | 4.8611837E-6 | 40.0 | 24 |
GTGTTGC | 40 | 1.9239451E-8 | 40.0 | 30 |
CTTAAGA | 80 | 0.0 | 40.0 | 14 |
TCGCCCT | 20 | 0.001254989 | 40.0 | 15 |
TCGCCCC | 20 | 0.001254989 | 40.0 | 30 |
TTCTATG | 30 | 4.8611837E-6 | 40.0 | 15 |
ACAACGC | 45 | 1.2187229E-9 | 40.0 | 25 |
ATCCTGT | 45 | 1.2187229E-9 | 40.0 | 28 |
GTTTGGT | 20 | 0.001254989 | 40.0 | 19 |
AAACTTT | 30 | 4.8611837E-6 | 40.0 | 22 |