FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_85.3510000004f8a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_85.3510000004f8a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461820
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA21900.47421073145381315Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA20910.45277380797713396No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA5650.12234203802347235No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA5620.12169243428175479No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA5490.11887748473431206No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG5260.11389718938114417No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA5120.11086570525312893No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG4980.10783422112511368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT257.787405E-540.015
TCCGGAA200.00125498940.015
TCGCCTA304.8611837E-640.015
ATTTATT257.787405E-540.027
ATTTATA555.456968E-1240.024
ACAACTA200.00125498940.024
GTTTGTT600.040.024
GTTTGTC200.00125498940.030
ATTTAGG353.051082E-740.018
GTTGAGG200.00125498940.025
GTTGAGA304.8611837E-640.024
GTGTTGC401.9239451E-840.030
CTTAAGA800.040.014
TCGCCCT200.00125498940.015
TCGCCCC200.00125498940.030
TTCTATG304.8611837E-640.015
ACAACGC451.2187229E-940.025
ATCCTGT451.2187229E-940.028
GTTTGGT200.00125498940.019
AAACTTT304.8611837E-640.022