FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_84.3510000004f898.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_84.3510000004f898.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274442
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA11670.4252264595069268No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA9070.3304887735842182Illumina PCR Primer Index 11 (96% over 28bp)
CAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTG3680.13409026315214143No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3470.12643837313530726No Hit
CGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACT3390.12352336741460856No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3030.11040584167146428No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG3020.11004146595637694No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA2940.10712646023567822No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG2890.10530458166024151No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA2840.1034827030848048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT401.9190338E-840.00000423
CCCTGAC200.001254098840.00000430
TCTGCCT1600.040.00000413
GTTTGTT200.001254098840.00000424
GTTGAGG200.001254098840.00000425
TCGCCCT200.001254098840.00000415
ACAACGC401.9190338E-840.00000425
TGTTTCC200.001254098840.00000430
TGTTTAT200.001254098840.00000421
CTATGTA800.040.00000414
TTAGGTC200.001254098840.00000415
TTAGGTA401.9190338E-840.00000415
TATCAAT200.001254098840.00000428
ATGGGCT200.001254098840.00000421
CGGCGTC200.001254098840.00000429
GAGGAGC200.001254098840.00000420
AACAAGC200.001254098840.00000424
TTAGGAG200.001254098840.00000425
GAGGAAG200.001254098840.00000426
AACAACC200.001254098840.00000424
Produced by FastQC (version 0.11.2)