FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_83.3510000004f88b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_83.3510000004f88b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247843
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA88423.5675810896414264Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA10600.4276901102714219No Hit
GGCGTTATAACCTCACACTCAATCTTTTATCACGAAGTCATGATTG4320.17430389399740964No Hit
GCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTACCGCTTC3700.14928805735889253No Hit
CTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATC3410.1375871015118442No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2940.11862348341490378No Hit
CATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAA2930.11822000217879867No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA2890.11660607723437823No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2850.11499215228995775No Hit
GATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCC2800.11297474610943217No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA2760.11136082116501173No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA2720.10974689622059126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT304.8520506E-640.00000415
CCTTCGC200.001253863640.00000428
ATTTATT200.001253863640.00000427
ATTTAGG200.001253863640.00000418
CTTAAGT200.001253863640.00000414
GTTGAGA401.9177605E-840.00000424
ACAACGC304.8520506E-640.00000425
GTGTTCC304.8520506E-640.00000430
GTCATGA401.9177605E-840.00000437
ATTTAAC200.001253863640.00000412
CTTCGGG200.001253863640.00000418
ATCCTCC304.8520506E-640.00000430
GTTTGCG304.8520506E-640.00000431
TTTAGAT200.001253863640.00000427
GAGGATG200.001253863640.00000423
GAGTGTA401.9177605E-840.00000433
AAACTGA200.001253863640.00000425
AGCAATT200.001253863640.00000423
CTAGAAT200.001253863640.00000414
GGGTGTA200.001253863640.00000433
Produced by FastQC (version 0.11.2)