FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_82.3510000004f87e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_82.3510000004f87e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274639
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA52031.8944869446801076Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA12240.4456759600785031No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3060.11141899001962577No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA2970.10814196090140146No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2900.10559316047611592No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2900.10559316047611592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA200.001254100540.00000422
TCCGGCT200.001254100540.00000415
TCCGGAT200.001254100540.00000415
TCCGGAA200.001254100540.00000415
ATTTATT200.001254100540.00000427
CTTAATG200.001254100540.00000414
CCTTCCC200.001254100540.00000422
ATTTAGG200.001254100540.00000418
TCGCCCC200.001254100540.00000430
ATTTACT200.001254100540.00000425
ATTTACC401.9192157E-840.00000421
ACAACGC200.001254100540.00000425
ATCCTGT200.001254100540.00000428
TTAGGTT200.001254100540.00000428
CAGAATC200.001254100540.00000424
CAGCGTG200.001254100540.00000426
CGGGTGT200.001254100540.00000428
GAAATGA200.001254100540.00000424
AACGTAA200.001254100540.00000420
GGGTGTT200.001254100540.00000429
Produced by FastQC (version 0.11.2)