FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_81.3510000004f861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_81.3510000004f861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4836
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCG61812.779156327543426No Hit
AAGCAATATCAGCACCAACAGAAACAACCTGATTAGCGGCGTTGAC49310.194375516956162No Hit
GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTC3697.6302729528535975No Hit
CATCATAGCCAGATGCCCAGAGATTAGAGCGCATGACAAGTAAAGG3687.6095947063689No Hit
AAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCG3366.9478908188585615No Hit
GTTGGGGGAGCACATTGTAGCATTGTGCCAATTCATCCATTAACTT3086.368899917287014No Hit
TACCATGAAAAATATCAACCACACCAGAAGCAGCATCAGTGACGAC3066.327543424317618No Hit
CCACACCAGAAGCAGCATCAGTGACGACATTAGAAATATCCTTTGC2715.603804797353185No Hit
AATATAACGTTGACGATGTAGCTTTAGGTGTCTGTAAAACAGGTGC2695.562448304383788No Hit
CAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACACCA2284.714640198511166No Hit
GCAACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTC2144.425144747725393No Hit
AGAAATGACTCGCAAGGTTAGTGCTGAGGTTGACTTAGTTCATCAG1853.825475599669148No Hit
CTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGC1853.825475599669148No Hit
ACGCTGAATAGCAAAGCCTCTACGCGATTTCATAGTGGAGGCCTCC1793.70140612076096No Hit
GATAATTTTTCGACTCATCAGAAATATCCGAAAGTGTTAACTTCTG1773.660049627791563No Hit
GATGATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTT1563.225806451612903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGT256.695036E-540.012
AGCCTGC256.695036E-540.029
CTTTTCG457.694325E-1040.021
TGGTTTC256.695036E-540.015
AACGTTG352.2970926E-740.06
TAGCGGC303.931669E-640.034
CCAATTC303.931669E-640.028
AACGTGA650.040.019
GTGTCTG352.2970926E-740.028
GTATGCA457.694325E-1040.027
CATTGTG303.931669E-640.021
CATTGTA303.931669E-640.013
GTTGAGT457.694325E-1040.038
AACGTAC256.695036E-540.036
GTTGACG352.2970926E-740.09
TTCATCC303.931669E-640.032
AAGTAAA551.8189894E-1240.038
GAGCGCA551.8189894E-1240.027
CATGACA551.8189894E-1240.032
CCAACCA650.040.011
Produced by FastQC (version 0.11.2)