Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-AKRU9_l01n01_exp163gap1bff_80.3510000004f855.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1020 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTT | 323 | 31.666666666666664 | No Hit |
GACGGCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGAT | 245 | 24.019607843137255 | No Hit |
AAATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGC | 157 | 15.392156862745098 | No Hit |
TCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA | 101 | 9.901960784313726 | RNA PCR Primer, Index 41 (96% over 30bp) |
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 3 | 0.29411764705882354 | Illumina PCR Primer Index 11 (96% over 28bp) |
AGCTACAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTT | 3 | 0.29411764705882354 | No Hit |
TCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAAA | 3 | 0.29411764705882354 | Illumina PCR Primer Index 11 (96% over 26bp) |
GACGCCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGAT | 2 | 0.19607843137254902 | No Hit |
GTCCACGAGGTCTCTTCGCGGACCAGATTACTTAGCGTACGCTGCA | 2 | 0.19607843137254902 | No Hit |
GACGTCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGAT | 2 | 0.19607843137254902 | No Hit |
GTCCACGAGGTCTCTCATGATATGAAGTTTCCGCCGTACGCTGCAG | 2 | 0.19607843137254902 | No Hit |
GTCCACGAGGTCTCTATCACATAGAGAGACGCTGCCGTACGCTGCA | 2 | 0.19607843137254902 | No Hit |
ACATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGC | 2 | 0.19607843137254902 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATGTT | 20 | 7.456525E-4 | 40.000004 | 33 |
CGTCAGG | 20 | 7.456525E-4 | 40.000004 | 8 |
CCAATTG | 20 | 7.456525E-4 | 40.000004 | 27 |
TTCATGC | 20 | 7.456525E-4 | 40.000004 | 40 |
AACCGTC | 20 | 7.456525E-4 | 40.000004 | 5 |
TGGCAGA | 20 | 7.456525E-4 | 40.000004 | 39 |
TCCCAAT | 20 | 7.456525E-4 | 40.000004 | 25 |
GCCTTGA | 20 | 7.456525E-4 | 40.000004 | 31 |
GGGTTAG | 20 | 7.456525E-4 | 40.000004 | 14 |
GCCTCAT | 20 | 7.456525E-4 | 40.000004 | 5 |
TAGGAAC | 20 | 7.456525E-4 | 40.000004 | 18 |
TTGTATG | 20 | 7.456525E-4 | 40.000004 | 31 |
AGGATTG | 20 | 7.456525E-4 | 40.000004 | 12 |
CTCCCAA | 20 | 7.456525E-4 | 40.000004 | 24 |
ATTAGAG | 20 | 7.456525E-4 | 40.000004 | 25 |
TCAGGGT | 20 | 7.456525E-4 | 40.000004 | 11 |
TATGTTT | 20 | 7.456525E-4 | 40.000004 | 34 |
TCAGGAT | 20 | 7.456525E-4 | 40.000004 | 10 |
TGTTTTC | 20 | 7.456525E-4 | 40.000004 | 36 |
AGGGTTA | 20 | 7.456525E-4 | 40.000004 | 13 |