FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_80.3510000004f855.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_80.3510000004f855.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTT32331.666666666666664No Hit
GACGGCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGAT24524.019607843137255No Hit
AAATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGC15715.392156862745098No Hit
TCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1019.901960784313726RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA30.29411764705882354Illumina PCR Primer Index 11 (96% over 28bp)
AGCTACAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTT30.29411764705882354No Hit
TCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAAA30.29411764705882354Illumina PCR Primer Index 11 (96% over 26bp)
GACGCCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGAT20.19607843137254902No Hit
GTCCACGAGGTCTCTTCGCGGACCAGATTACTTAGCGTACGCTGCA20.19607843137254902No Hit
GACGTCCTCATCAGGGTTAGGAACATTAGAGCCTTGAATGGCAGAT20.19607843137254902No Hit
GTCCACGAGGTCTCTCATGATATGAAGTTTCCGCCGTACGCTGCAG20.19607843137254902No Hit
GTCCACGAGGTCTCTATCACATAGAGAGACGCTGCCGTACGCTGCA20.19607843137254902No Hit
ACATAACCGTCAGGATTGACACCCTCCCAATTGTATGTTTTCATGC20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTT207.456525E-440.00000433
CGTCAGG207.456525E-440.0000048
CCAATTG207.456525E-440.00000427
TTCATGC207.456525E-440.00000440
AACCGTC207.456525E-440.0000045
TGGCAGA207.456525E-440.00000439
TCCCAAT207.456525E-440.00000425
GCCTTGA207.456525E-440.00000431
GGGTTAG207.456525E-440.00000414
GCCTCAT207.456525E-440.0000045
TAGGAAC207.456525E-440.00000418
TTGTATG207.456525E-440.00000431
AGGATTG207.456525E-440.00000412
CTCCCAA207.456525E-440.00000424
ATTAGAG207.456525E-440.00000425
TCAGGGT207.456525E-440.00000411
TATGTTT207.456525E-440.00000434
TCAGGAT207.456525E-440.00000410
TGTTTTC207.456525E-440.00000436
AGGGTTA207.456525E-440.00000413