FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_8.3510000004f3d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_8.3510000004f3d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26235
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA8203.125595578425767Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC3311.2616733371450353No Hit
CCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATG2791.0634648370497428No Hit
CGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTA2290.8728797408042691No Hit
GATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCA2130.8118925100057175No Hit
TGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAG1950.7432818753573471No Hit
TTCAACCAGATATTGAAGCAGAACGCAAAAAGAGAGATGAGATTGA1930.7356584715075282No Hit
ACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGG1880.7165999618829808No Hit
GGCAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCG1730.6594244330093386No Hit
AGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA1340.5107680579378693No Hit
ACTGAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCATCTCGGC1310.49933295216314083No Hit
CTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGA1200.45740423098913663No Hit
AAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCG940.3582999809414904No Hit
CCAAAACGTCGGCTACAGTAACTTTTCCCAGCCTCAATCTCATCTC890.339241471316943No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA690.2630074328187536No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG620.23632551934438728No Hit
GTCCACGAGGTCTCTCTACGTCGGCTCATAGTCGTCGTACGCTGCA620.23632551934438728No Hit
TTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGA560.21345530779493047No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA480.18296169239565466No Hit
GTCCACGAGGTCTCTGCCCATGCTATAGATGATCCGTACGCTGCAG460.17533828854583572No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG430.1639031827711073No Hit
GTCCACGAGGTCTCTAGAGTGTCCCAAGTTCCCTTCGTACGCTGCA410.15627977892128836No Hit
GTCCACGAGGTCTCTGGAGTACCAACATATCCCGACGTACGCTGCA400.15246807699637888No Hit
GTCCACGAGGTCTCTTAAGTCTTCACAAGCCGTCACGTACGCTGCA370.14103297122165045No Hit
GTCCACGAGGTCTCTCGGACGGTATTCATTCATCTCGTACGCTGCA370.14103297122165045No Hit
GTCCACGAGGTCTCTCACGGTTATCAGATGTGGATCGTACGCTGCA360.13722126929674097No Hit
GTCCACGAGGTCTCTGAGTATCGGGCATTTCCGCTCGTACGCTGCA350.13340956737183152No Hit
GTCCACGAGGTCTCTAGCCCAACTCGTGTAAAGACGTACGCTGCAG340.12959786544692206No Hit
GTCCACGAGGTCTCTGCGCAAATAAAGATGTCGTGCGTACGCTGCA340.12959786544692206No Hit
GTCCACGAGGTCTCTGCGCAGAAGGCAATGCTATACGTACGCTGCA330.12578616352201258No Hit
GTCCACGAGGTCTCTGATACATGCACAATGCCGCACGTACGCTGCA320.12197446159710312No Hit
GTCCACGAGGTCTCTGGCATTACATCAAGATCCACCGTACGCTGCA310.11816275967219364No Hit
GTCCACGAGGTCTCTGGGATCACAACCACGTTACACGTACGCTGCA300.11435105774728416No Hit
GTCCACGAGGTCTCTTAGTACGGGTCAGTCTCTGTCGTACGCTGCA300.11435105774728416No Hit
GTCCACGAGGTCTCTTGGCATATCACAACAGTCCACGTACGCTGCA300.11435105774728416No Hit
GTCCACGAGGTCTCTAATGCACGGGCACCCTGTATCGTACGCTGCA300.11435105774728416No Hit
GTCCACGAGGTCTCTGCGATCAGAACAGATGCCGACGTACGCTGCA290.11053935582237469No Hit
GTCCACGAGGTCTCTATGAGAGCCGGATCGTCCTTCGTACGCTGCA290.11053935582237469No Hit
GTCCACGAGGTCTCTATGCTACGGCCACTAGGTTGCGTACGCTGCA290.11053935582237469No Hit
GTCCACGAGGTCTCTCTGAAGGAGCAATTTCGAGACGTACGCTGCA290.11053935582237469No Hit
GTCCACGAGGTCTCTACACACTGTATAGAGGGTTGCGTACGCTGCA290.11053935582237469No Hit
GTCCACGAGGTCTCTAACGCATACTCAGTCGGAGGCGTACGCTGCA290.11053935582237469No Hit
GTCCACGAGGTCTCTAGATGATCCACAGCATTGCCCGTACGCTGCA290.11053935582237469No Hit
GTCCACGAGGTCTCTTCAGAAGCCCAGCTTTGTACGTACGCTGCAG290.11053935582237469No Hit
GTCCACGAGGTCTCTAGACTGACGCAAGGCACCTACGTACGCTGCA290.11053935582237469No Hit
GTCCACGAGGTCTCTTCGTATAGCAAACGGGCGACGTACGCTGCAG290.11053935582237469No Hit
GTCCACGAGGTCTCTACTACTGGCTTAGATGTGGTCGTACGCTGCA290.11053935582237469No Hit
GTCCACGAGGTCTCTACGAGAGCCACGCATGTTAGCGTACGCTGCA280.10672765389746523No Hit
GTCCACGAGGTCTCTATCCATTGTGTCTAAGAGGGCGTACGCTGCA280.10672765389746523No Hit
GTCCACGAGGTCTCTACACACATGAAGGGATTCCCGTACGCTGCAG280.10672765389746523No Hit
GTCCACGAGGTCTCTGGGACGATCACATTCCTATCCGTACGCTGCA270.10291595197255575No Hit
GTCCACGAGGTCTCTCATCTGATAGCATTGCGGTGCGTACGCTGCA270.10291595197255575No Hit
GTCCACGAGGTCTCTGCCAGACGGACAATGTTAGACGTACGCTGCA270.10291595197255575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAT200.001233447140.05
CTCTGGT257.587859E-540.012
CTCTGGG401.8078936E-840.012
CTCTGGC507.094059E-1140.012
CTCTGGA352.9067996E-740.012
CGCGAAC200.001233447140.022
CTACACT200.001233447140.014
CTCTGCT352.9067996E-740.012
GGATGTT451.1277734E-940.018
CTCTGCG451.1277734E-940.012
CTCTGCC900.040.012
CTCTGAT600.040.012
CTCTGAC257.587859E-540.012
ACAGTAT304.6877267E-640.039
ACACGTA257.587859E-540.033
GGTGATT451.1277734E-940.034
GAGGTCG800.040.07
ACTGTCG700.040.027
AAACCGT200.001233447140.032
TGGTTAC304.6877267E-640.034
Produced by FastQC (version 0.11.2)