FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_79.3510000004f848.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_79.3510000004f848.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3197
Sequences flagged as poor quality0
Sequence length46
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGCTGTACCATACTCAGGCACACAAAAATACTGATAGCAGTCGG45514.232092586800125No Hit
TAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAG42113.16859555833594No Hit
CGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGA36211.323115420706912No Hit
CAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGCG35711.166718798873944No Hit
CGACGCAATGGAGAAAGACGGAGAGCGCCAACGGCGTCCATCTCGA2006.255864873318736No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1594.973412574288395Illumina PCR Primer Index 11 (96% over 28bp)
AAGCGGTCTGGAAACGTACGGATTGTTCAGTAACTTGACTCATGAT942.9402564904598063No Hit
GTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGCCGCGT692.158273381294964No Hit
GTGTCGAGCTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA100.31279324366593686RNA PCR Primer, Index 27 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA60.1876759461995621No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA50.15639662183296843RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTAATGCCTTGAATTAGGGCGTGTACGCTGCAG40.12511729746637473No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG40.12511729746637473No Hit
GTCCACGAGGTCTCTCACAGCTAAACTCACGGACGTACGCTGCAGG40.12511729746637473No Hit
GTCCACGAGGTCTCTAGCCAATTACAATGTGACGTACGCTGCAGGT40.12511729746637473No Hit
GTCCACGAGGTCTCTCATGAGGGCAAAGTCCTGAACGTACGCTGCA40.12511729746637473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAG351.9635081E-740.022
GGAGAGC200.00107472340.020
TAGCGAA455.9480953E-1040.035
TTTTGCA200.00107472340.05
GTTTGTA351.9635081E-740.028
GGAGAAA200.00107472340.010
AACGTGA455.9480953E-1040.012
ACAAAAA800.040.024
CAATGGA200.00107472340.06
GAGAAAG200.00107472340.011
GAGCGCC200.00107472340.023
CCAACGG200.00107472340.028
CCAACCA455.9480953E-1040.04
TTGCAAG200.00107472340.07
CAGTCGG800.040.040
ATCTCGA200.00107472340.040
CGGAGAG200.00107472340.019
GGCACAC800.040.019
CACACAA800.040.021
ACTGATA800.040.032
Produced by FastQC (version 0.11.2)