000000000-AKRU9_l01n01_exp163gap1bff_78.3510000004f83b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_78.3510000004f83b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375752
Sequences flagged as poor quality	0
Sequence length	46
%GC	56

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	12850	3.419808810066214	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	1676	0.44603887670591236	No Hit
GACTTTTTGTTAACGTATTTAGCCACATAGAAACCAACAGCCATAT	445	0.11842917669100896	No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	409	0.10884838936319699	No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA	402	0.10698545849390023	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	398	0.1059209265685878	No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG	385	0.10246119781132236	No Hit
TGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCT	379	0.1008643999233537	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	25	7.784627E-5	40.000004	15
GTTTGTA	25	7.784627E-5	40.000004	33
GTTGAGA	25	7.784627E-5	40.000004	24
TCGCCCT	25	7.784627E-5	40.000004	15
TATTTCT	25	7.784627E-5	40.000004	16
ACAACGT	25	7.784627E-5	40.000004	32
CAGAAGA	25	7.784627E-5	40.000004	23
GGGTGAG	25	7.784627E-5	40.000004	25
GGGTGAC	25	7.784627E-5	40.000004	30
CGGCGAA	25	7.784627E-5	40.000004	27
GGTACCG	50	7.6397555E-11	40.000004	31
TACCCCG	50	7.6397555E-11	40.000004	31
CCGGGCG	25	7.784627E-5	40.000004	31
GGAGCCG	50	7.6397555E-11	40.000004	31
GATGTTG	25	7.784627E-5	40.000004	19
CGTATTG	25	7.784627E-5	40.000004	17
CCCTCAT	25	7.784627E-5	40.000004	29
AGTTTAA	50	7.6397555E-11	40.000004	21
AAGAGTC	25	7.784627E-5	40.000004	26
CTCCGGA	50	7.6397555E-11	40.000004	14