FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_77.3510000004f82e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_77.3510000004f82e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321537
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA61051.8986928409483201Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA13510.4201693739756233No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG4750.1477279442179283No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3890.12098141115952442No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3700.11507229339080727No Hit
GTCCACGAGGTCTCTCTACGTCGGCTCATAGTCGTCGTACGCTGCA3490.10854116322538308No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA3410.10605311363855482No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA3400.10574210744020128No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG3280.10201003305995889No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA3250.1010770144648983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA257.7821154E-540.00000422
TCCGGCC257.7821154E-540.00000418
ATTTATC257.7821154E-540.00000417
TCTGCCT2000.040.00000413
TCGCCCG507.6397555E-1140.00000431
ACAACGC507.6397555E-1140.00000425
GTGTTCA257.7821154E-540.00000422
ATTTAAC257.7821154E-540.00000420
GTTGAAA257.7821154E-540.00000421
GTTTGAT257.7821154E-540.00000429
TTAGGTC257.7821154E-540.00000415
CAGAATA257.7821154E-540.00000423
AACGTGG257.7821154E-540.00000419
AAACTCT257.7821154E-540.00000425
TGGAGTC257.7821154E-540.00000415
GGAATCC257.7821154E-540.00000424
TCGTTTA257.7821154E-540.00000419
GAGTGAA257.7821154E-540.00000421
TGGAGGT257.7821154E-540.00000417
GGGTGCG257.7821154E-540.00000431
Produced by FastQC (version 0.11.2)