FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_76.3510000004f811.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_76.3510000004f811.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328540
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA93892.8577950934437206Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA13630.4148657697692823No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3810.11596761429354112No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3790.11535886041273513No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG3400.10348815973701832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAA257.78249E-540.015
CTTAATG257.78249E-540.014
TTGATTT200.001254460240.030
GTGTTTA257.78249E-540.028
CCTTCCG900.040.031
CCTTCAA200.001254460240.022
CTTGTCT257.78249E-540.029
GTTTGTT401.9210347E-840.024
GTTTGTA200.001254460240.033
CTTGTAT353.0474985E-740.014
CTTAAGT200.001254460240.014
GTTGAGG257.78249E-540.025
GTGTTGC200.001254460240.030
CTTAAGA257.78249E-540.014
ACAACGC200.001254460240.025
ATCCTGT353.0474985E-740.028
CTTAAAT257.78249E-540.022
TGTTTGA257.78249E-540.028
ATGGGTG200.001254460240.027
AGCGTTT200.001254460240.016
Produced by FastQC (version 0.11.2)