FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_75.3510000004f804.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_75.3510000004f804.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186238
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA26231.4084128910319054Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA7960.4274100881667544No Hit
CCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAA4880.2620303053082615No Hit
ACGCCATTAATAATGTTTTCCGTAAATTCAGCGCCTTCCATGATGA4500.24162630612442143No Hit
GCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCC2650.14229104693993705No Hit
TCGCTGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTTTGTAGGA2440.13101515265413075No Hit
CGTTGCGTCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAG2370.12725652122552863No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG2140.11490673224583596No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2100.11275894285806334No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA2060.11061115347029071No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2050.11007420612334753No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA2000.10738946938863175No Hit
TCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGG1870.10040915387837068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA200.001253060840.00000423
TCAAAAA200.001253060840.00000424
TCTGCCC1600.040.00000413
GTTTGTT200.001253060840.00000424
TGTTTTC600.040.00000414
GTGTTGT200.001253060840.00000423
CAGGTTG200.001253060840.00000419
CTTAAGA200.001253060840.00000414
ACAACGC200.001253060840.00000425
GTTGACG200.001253060840.00000431
CTGCTCT200.001253060840.00000414
ATCCTGT200.001253060840.00000428
CTAGATT200.001253060840.00000414
GTTTGCG200.001253060840.00000431
GTCATAC200.001253060840.00000425
GTTTGAT200.001253060840.00000429
TGTTTAG304.8455404E-640.00000429
GAGGATG200.001253060840.00000423
GTGCACG304.8455404E-640.00000431
AGCAATT200.001253060840.00000423
Produced by FastQC (version 0.11.2)