FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_74.3510000004f7f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_74.3510000004f7f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243588
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA123095.05320459135918Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA9990.4101187250603478No Hit
ATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCAT4310.17693810860961953No Hit
CTTCGGCGTTATAACCTCACACTCAATCTTTTATCACGAAGTCATG4090.16790646501469694No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA3370.13834835870404125RNA PCR Primer, Index 41 (96% over 28bp)
CCAGCAATAGCACCAAACATAAATCACCTCACTTAAGTGGCTGGAG3330.13670624168678258No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA2980.12233771778576942No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2780.11412713269947616No Hit
CGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTAC2750.11289554493653217No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA2660.10920078164770022No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2580.10591654761318292No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG2470.10140072581572163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC304.8517068E-640.00000425
CTGCTCT304.8517068E-640.00000414
CAGCGGT304.8517068E-640.00000428
GGGTGCG304.8517068E-640.00000431
TTGGCGT600.040.00000432
CCCCTCA304.8517068E-640.00000417
GCAAGAA304.8517068E-640.00000424
AATTTTT304.8517068E-640.00000438
AGTTTAA304.8517068E-640.00000421
CAAGTGA304.8517068E-640.00000416
TTGTGCT304.8517068E-640.00000417
CAAAAAT304.8517068E-640.00000428
ACCATTG304.8517068E-640.00000424
AACCCCT304.8517068E-640.00000415
CCGGCTA304.8517068E-640.00000416
CTTTTAG304.8517068E-640.00000414
ATAATAT304.8517068E-640.00000425
ATGGAAA304.8517068E-640.00000421
TATGGAT304.8517068E-640.00000417
GCAGTTC304.8517068E-640.00000425