Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-AKRU9_l01n01_exp163gap1bff_74.3510000004f7f6.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 243588 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 12309 | 5.05320459135918 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA | 999 | 0.4101187250603478 | No Hit |
ATAACAATACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCAT | 431 | 0.17693810860961953 | No Hit |
CTTCGGCGTTATAACCTCACACTCAATCTTTTATCACGAAGTCATG | 409 | 0.16790646501469694 | No Hit |
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 337 | 0.13834835870404125 | RNA PCR Primer, Index 41 (96% over 28bp) |
CCAGCAATAGCACCAAACATAAATCACCTCACTTAAGTGGCTGGAG | 333 | 0.13670624168678258 | No Hit |
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA | 298 | 0.12233771778576942 | No Hit |
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA | 278 | 0.11412713269947616 | No Hit |
CGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAATTAAAATTTTTAC | 275 | 0.11289554493653217 | No Hit |
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA | 266 | 0.10920078164770022 | No Hit |
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG | 258 | 0.10591654761318292 | No Hit |
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG | 247 | 0.10140072581572163 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACAACGC | 30 | 4.8517068E-6 | 40.000004 | 25 |
CTGCTCT | 30 | 4.8517068E-6 | 40.000004 | 14 |
CAGCGGT | 30 | 4.8517068E-6 | 40.000004 | 28 |
GGGTGCG | 30 | 4.8517068E-6 | 40.000004 | 31 |
TTGGCGT | 60 | 0.0 | 40.000004 | 32 |
CCCCTCA | 30 | 4.8517068E-6 | 40.000004 | 17 |
GCAAGAA | 30 | 4.8517068E-6 | 40.000004 | 24 |
AATTTTT | 30 | 4.8517068E-6 | 40.000004 | 38 |
AGTTTAA | 30 | 4.8517068E-6 | 40.000004 | 21 |
CAAGTGA | 30 | 4.8517068E-6 | 40.000004 | 16 |
TTGTGCT | 30 | 4.8517068E-6 | 40.000004 | 17 |
CAAAAAT | 30 | 4.8517068E-6 | 40.000004 | 28 |
ACCATTG | 30 | 4.8517068E-6 | 40.000004 | 24 |
AACCCCT | 30 | 4.8517068E-6 | 40.000004 | 15 |
CCGGCTA | 30 | 4.8517068E-6 | 40.000004 | 16 |
CTTTTAG | 30 | 4.8517068E-6 | 40.000004 | 14 |
ATAATAT | 30 | 4.8517068E-6 | 40.000004 | 25 |
ATGGAAA | 30 | 4.8517068E-6 | 40.000004 | 21 |
TATGGAT | 30 | 4.8517068E-6 | 40.000004 | 17 |
GCAGTTC | 30 | 4.8517068E-6 | 40.000004 | 25 |