FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_73.3510000004f7e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_73.3510000004f7e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524919
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA24060.4583564321352437No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA10660.20307895122866573Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA7000.13335390793627208No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA6130.11677992223562111No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA5940.11316031616306517No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG5750.10954071009050921No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG5750.10954071009050921No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA5590.10649262076625156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTG200.001255145440.00000428
TCCGGCT600.040.00000415
TCCGGAT304.8624534E-640.00000415
TCGCCTA401.9248546E-840.00000415
CTTAATT200.001255145440.00000429
ATTTATA200.001255145440.00000424
CTTGTCC401.9248546E-840.00000414
CTTGTAT401.9248546E-840.00000414
ATTTAGT304.8624534E-640.00000427
GTTGAGG304.8624534E-640.00000425
ACAACGC800.040.00000425
TTGATCA200.001255145440.00000415
CATGGTT200.001255145440.00000420
GTTTGGA304.8624534E-640.00000429
ACAACAT200.001255145440.00000425
AGCGTTT304.8624534E-640.00000416
GTTTGCG401.9248546E-840.00000431
TGTTTCC304.8624534E-640.00000430
ATCCTAC401.9248546E-840.00000422
CGTGGTA200.001255145440.00000419