Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-AKRU9_l01n01_exp163gap1bff_73.3510000004f7e9.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 524919 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 56 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA | 2406 | 0.4583564321352437 | No Hit |
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 1066 | 0.20307895122866573 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA | 700 | 0.13335390793627208 | No Hit |
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA | 613 | 0.11677992223562111 | No Hit |
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA | 594 | 0.11316031616306517 | No Hit |
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG | 575 | 0.10954071009050921 | No Hit |
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG | 575 | 0.10954071009050921 | No Hit |
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA | 559 | 0.10649262076625156 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTGTTG | 20 | 0.0012551454 | 40.000004 | 28 |
TCCGGCT | 60 | 0.0 | 40.000004 | 15 |
TCCGGAT | 30 | 4.8624534E-6 | 40.000004 | 15 |
TCGCCTA | 40 | 1.9248546E-8 | 40.000004 | 15 |
CTTAATT | 20 | 0.0012551454 | 40.000004 | 29 |
ATTTATA | 20 | 0.0012551454 | 40.000004 | 24 |
CTTGTCC | 40 | 1.9248546E-8 | 40.000004 | 14 |
CTTGTAT | 40 | 1.9248546E-8 | 40.000004 | 14 |
ATTTAGT | 30 | 4.8624534E-6 | 40.000004 | 27 |
GTTGAGG | 30 | 4.8624534E-6 | 40.000004 | 25 |
ACAACGC | 80 | 0.0 | 40.000004 | 25 |
TTGATCA | 20 | 0.0012551454 | 40.000004 | 15 |
CATGGTT | 20 | 0.0012551454 | 40.000004 | 20 |
GTTTGGA | 30 | 4.8624534E-6 | 40.000004 | 29 |
ACAACAT | 20 | 0.0012551454 | 40.000004 | 25 |
AGCGTTT | 30 | 4.8624534E-6 | 40.000004 | 16 |
GTTTGCG | 40 | 1.9248546E-8 | 40.000004 | 31 |
TGTTTCC | 30 | 4.8624534E-6 | 40.000004 | 30 |
ATCCTAC | 40 | 1.9248546E-8 | 40.000004 | 22 |
CGTGGTA | 20 | 0.0012551454 | 40.000004 | 19 |