FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_72.3510000004f7dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_72.3510000004f7dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215418
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA99544.620783778514331Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC29931.3893917871301378No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA8680.4029375446805745No Hit
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA7540.35001717590916265RNA PCR Primer, Index 41 (96% over 30bp)
ATAGTCACACAGTCCTTGACGGTATAATAACCACCATCATGGCGAC4240.1968266347287599No Hit
TTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCAT4030.18707814574455245No Hit
ACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGA3840.17825808428265047No Hit
ACATACATATCACCATTATCGAACTCAACGCCCTGCATACGAAAAG3700.17175909162651218No Hit
AACTATCAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGTAA3550.16479588520922114No Hit
TAAGAGGTTTTACCTCCAAATGAAGAAATAACATCATGGTAACGCT3140.14576312100195898No Hit
GTAAGAACGTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGA3000.1392641283458207No Hit
TTTGGCGAGAAAGCTCAGTCTCAGGAGGAAGCGGAGCAGTCCAAAT2870.13322934945083512No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA2670.12394507422778041RNA PCR Primer, Index 41 (96% over 28bp)
CGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAAT2560.11883872285510032No Hit
GAAAATCGAAATCATCTTCGGTTAAATCCAAAACGGCAGAAGCCTG2460.11419658524357296No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA2310.10723337882628194No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG2270.10537652378167098No Hit
AGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA2180.10119859993129637No Hit
GTCCACGAGGTCTCTAGACGTGATTCAGCTCTCTTCGTACGCTGCA2180.10119859993129637No Hit
GTCCACGAGGTCTCTCTACGTCGGCTCATAGTCGTCGTACGCTGCA2170.10073438617014363No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA2160.10027017240899089No Hit
AAAATTACGTGCAGAAGGAGTGATGTAATGTCTAAAGGTAAAAAAC2160.10027017240899089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAAT353.0409683E-740.06
TCAAAAA200.001253498740.016
ACGCGAC304.849091E-640.030
CGGACAA304.849091E-640.022
GTGTTTA200.001253498740.028
CCTTCCG401.9157596E-840.031
TGTTTTC200.001253498740.024
TATCATT200.001253498740.015
GTGTTGC257.7735516E-540.030
CTTAAGA257.7735516E-540.014
TCGCCCG507.6397555E-1140.031
ACAACGT401.9157596E-840.032
ACAACGC353.0409683E-740.025
AACGTTG353.0409683E-740.015
TTGATCA257.7735516E-540.015
CTAGATT257.7735516E-540.014
TTTAGGT353.0409683E-740.032
TGTTTGA257.7735516E-540.028
CTTAAAC257.7735516E-540.022
GTGTTAA353.0409683E-740.039
Produced by FastQC (version 0.11.2)