FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_71.3510000004f7cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_71.3510000004f7cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35925
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGCTCCTAGACCTTTA3631.010438413361169No Hit
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC3621.0076548364648572No Hit
ATGTATCCATCTGAATGCAATGAAGAAAACCACCATTACCAGCATT3020.8406402226861518No Hit
GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGG2980.8295059151009045No Hit
AATCTCTTCCAAGAGCTTGATGCGGTTATCCATCTGCTTATGGAAG1920.5344467640918581No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1710.47599164926931103Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA1580.43980514961725814No Hit
GCCGTTTGAATGTTGACGGGATGAACATAATAAGCAATGACGGCAG1550.43145441892832287No Hit
ATTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCATTCTG1190.3312456506610995No Hit
AATCGTTAGTTGATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAG880.24495476687543494No Hit
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA810.2254697286012526No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA670.18649965205288796No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA670.18649965205288796No Hit
GTCCACGAGGTCTCTCTACGTCGGCTCATAGTCGTCGTACGCTGCA650.18093249826026445No Hit
GTCCACGAGGTCTCTCCGGTTGCCAAAGCGAGAAACGTACGCTGCA540.1503131524008351No Hit
GTCCACGAGGTCTCTGATGCTGACACATGATGCTCCGTACGCTGCA490.13639526791927625No Hit
GTCCACGAGGTCTCTGCGACATACACACAGCGGTACATACGCTGCA490.13639526791927625No Hit
GTCCACGAGGTCTCTCGGTAACGTATAATCCTGTCCGTACGCTGCA470.13082811412665274No Hit
GTCCACGAGGTCTCTCCTAGAGGCTAATTTGCATCCGTACGCTGCA460.12804453723034098No Hit
GTCCACGAGGTCTCTAGATGGCTGCTCATGCTCCTCGTACGCTGCA460.12804453723034098No Hit
GTCCACGAGGTCTCTCATAGCGTACAATGCTCGACGTACGCTGCAG460.12804453723034098No Hit
GTCCACGAGGTCTCTTCGCGGACCAGATTACTTAGCGTACGCTGCA460.12804453723034098No Hit
GTCCACGAGGTCTCTAGAGGTATAACACTCACCCCGTACGCTGCAG450.12526096033402923No Hit
GTCCACGAGGTCTCTGCGACTTACAGCACTATCAGTGTACGCTGCA440.12247738343771747No Hit
GTCCACGAGGTCTCTACCCAGTAGTGAACAGGAGCCGTACGCTGCA440.12247738343771747No Hit
GTCCACGAGGTCTCTTAAGCTAGTATAACCGCTGCCGTACGCTGCA440.12247738343771747No Hit
GTCCACGAGGTCTCTTAACGGAGGCGAACTTAGCCGTACGCTGCAG430.1196938065414057No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG430.1196938065414057No Hit
GTCCACGAGGTCTCTGAGATGTGGATCTCACCCATCGTACGCTGCA430.1196938065414057No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA430.1196938065414057No Hit
GTCCACGAGGTCTCTTCATCGTCGCGCAGTTGTTGCGTACGCTGCA420.11691022964509395No Hit
GTCCACGAGGTCTCTCATCTACTCGCAGGATAGGGCGTACGCTGCA420.11691022964509395No Hit
GTCCACGAGGTCTCTGCTCGACATTAACAGACTACCGTACGCTGCA420.11691022964509395No Hit
GTCCACGAGGTCTCTGCCAAAGACAGATGCCATTCCGTACGCTGCA400.11134307585247043No Hit
GTCCACGAGGTCTCTCACGACTGTGAACTATCATCCGTACGCTGCA400.11134307585247043No Hit
GTCCACGAGGTCTCTAGATGTAGAGCATATACCGGCGTACGCTGCA400.11134307585247043No Hit
GTCCACGAGGTCTCTGATGCGGCACTATCGCTCATCGTACGCTGCA390.10855949895615866No Hit
GTCCACGAGGTCTCTAGCTATTCTCTCACATGGCTCGTACGCTGCA390.10855949895615866No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG390.10855949895615866No Hit
GTCCACGAGGTCTCTCCCGCAGACAGAATCATTTACGTACGCTGCA390.10855949895615866No Hit
GTCCACGAGGTCTCTGGCTAGAACCAATTCCCAGACGTACGCTGCA380.10577592205984691No Hit
GTCCACGAGGTCTCTCACTATACGTTGAGACCATGCGTACGCTGCA380.10577592205984691No Hit
GTCCACGAGGTCTCTGAGTCGGCTACACTCTATGCGTACGCTGCAG380.10577592205984691No Hit
GTCCACGAGGTCTCTACGAGGCAAGATATTTGGACGTACGCTGCAG370.10299234516353514No Hit
GTCCACGAGGTCTCTCCGAGTTATTGACATGAGTGCGTACGCTGCA360.10020876826722339No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA360.10020876826722339No Hit
GTCCACGAGGTCTCTTCTGACAGCAAATACGCTCACGTACGCTGCA360.10020876826722339No Hit
GTCCACGAGGTCTCTACGCTCGTATCACTGGTTATCGTACGCTGCA360.10020876826722339No Hit
GTCCACGAGGTCTCTTCAACACGCGGAAAGGTGCAGGTACGCTGCA360.10020876826722339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGA257.644629E-540.038
TGATGGT200.001239592140.09
CGTTTGA200.001239592140.03
TGATGGC200.001239592140.011
AGCCCGT257.644629E-540.032
CTCTGGT401.8404535E-840.012
CTCTGGG401.8404535E-840.012
CTCTGGC650.040.012
CTCTGGA451.1532393E-940.012
TGGTTTT257.644629E-540.03
TGGTTTA200.001239592140.016
CTCTGCT304.736916E-640.012
CTCTGAT1000.040.012
TTTGACA200.001239592140.028
TTTGAAT200.001239592140.05
GTTGATG200.001239592140.09
TTTTGAC200.001239592140.027
TGGTTGG200.001239592140.012
ACTGTCG553.6379788E-1240.027
TTGTTCC257.644629E-540.017
Produced by FastQC (version 0.11.2)