FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_70.3510000004f7b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_70.3510000004f7b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326855
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC33181.0151290327515259No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA18580.5684477826559178Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA17290.5289807406954155No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA5020.15358492297807896No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA4690.14348870294167138No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA4170.1275795077327867No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA4030.12329626286885621No Hit
AATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAGA3970.12146058649860031No Hit
GTCCACGAGGTCTCTGCGACATACACACAGCGGTACATACGCTGCA3730.1141178810175766No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG3510.10738706765997155No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG3480.10646922947484358No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA3430.10493949916629698No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG3350.10249193067262241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT257.7824E-540.00000415
CTTGTGG257.7824E-540.00000414
TCTGCAG1850.040.00000413
TTCTATG257.7824E-540.00000415
TGTTTGA507.6397555E-1140.00000428
ATCCTAC257.7824E-540.00000422
GTTTGAT507.6397555E-1140.00000429
AGGTATA257.7824E-540.00000417
GAAATCA257.7824E-540.00000424
AACAAGC257.7824E-540.00000424
CAGAAAT257.7824E-540.00000422
AACAACT507.6397555E-1140.00000434
AACAACG257.7824E-540.00000424
AGCAAGG257.7824E-540.00000427
CGGCGGA257.7824E-540.00000417
GCCTTCG507.6397555E-1140.00000431
TGCCCTA257.7824E-540.00000415
TCGCAAA257.7824E-540.00000428
ACACGGC257.7824E-540.00000427
GCAAGCT257.7824E-540.00000421
Produced by FastQC (version 0.11.2)