FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_7.3510000004f3c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_7.3510000004f3c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264460
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA4532417.138319594645694Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC25860.9778416395674203No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA11650.4405203055282463RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA11610.439007789457763No Hit
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA3830.14482341374877108RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA3350.1266732209029721RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTCCGGGAGCTTAATATACATCCGTACGCTGCA3260.12327005974438479No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA3010.11381683430386448Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA2990.11306057626862287No Hit
GTCCACGAGGTCTCTCCCTGCACATTGCGTAATATCGTACGCTGCA2860.1081448990395523No Hit
GTCCACGAGGTCTCTGAGACAGGAGCATATTGTACCGTATGCTGCA2800.10587612493382743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC200.001254016440.00000430
CGGACAA200.001254016440.00000422
TCCGGCT401.91867E-840.00000415
TCCGGAT200.001254016440.00000415
AGCACAA200.001254016440.00000422
ACAACTG200.001254016440.00000425
ATTTAGG200.001254016440.00000418
GTTGAGA200.001254016440.00000424
CTTAAGA401.91867E-840.00000414
TCGCCCG304.853291E-640.00000431
ATTTACC257.778364E-540.00000421
ACAACGC257.778364E-540.00000425
GTTGACG401.91867E-840.00000431
CTTCGGT200.001254016440.00000414
CTAGATA600.040.00000414
TTTAGGC304.853291E-640.00000415
ATCCTCC257.778364E-540.00000430
ACAACAG304.853291E-640.00000425
GTTTGCG304.853291E-640.00000431
ACAACAC200.001254016440.00000425
Produced by FastQC (version 0.11.2)