FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_69.3510000004f7a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_69.3510000004f7a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACTGCAACAACTGAACGGACTGGAAACACTGGTCATAATCATG32315.366317792578496No Hit
CAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGTAAAACAGG26412.559467174119888No Hit
CTGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGT24311.560418648905804No Hit
TTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGG22510.70409134157945No Hit
TTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATG21910.418648905803996No Hit
AGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAG21210.085632730732636No Hit
GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGACTAAAGAG1909.039010466222646No Hit
GAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATG1677.944814462416747No Hit
TGACGTCCTTCCCCGTACGCCGGGCAATAATGTTTATGTTGGTTTC1597.564224548049476No Hit
GTGTCGAGCTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA90.4281636536631779RNA PCR Primer, Index 27 (96% over 28bp)
GTGTCGGTCTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA60.285442435775452RNA PCR Primer, Index 27 (96% over 28bp)
TCCACTGCAACAACTGAACGGACTGGAAACACTGGTCATAATCACG30.142721217887726No Hit
GTTTACTCTTTCGCTTGTTCGTTTTCCGCCTACTGCGACTAAAGAG30.142721217887726No Hit
TTTAACGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATG30.142721217887726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG255.4487544E-540.09
ACTGCGA407.9835445E-940.032
TTGTTCG407.9835445E-940.015
GTGTCTG255.4487544E-540.031
GGATGCC302.9362564E-640.014
GTTGACG255.4487544E-540.012
CCTACTG407.9835445E-940.029
ACTAAAG407.9835445E-940.038
AGCTTTA255.4487544E-540.023
CCGTGGA302.9362564E-640.010
CTTTAGG255.4487544E-540.025
TGCGCTT407.9835445E-940.010
TTCGTTT407.9835445E-940.018
GCGACTA407.9835445E-940.035
TGACCGT302.9362564E-640.021
TTACTCT407.9835445E-940.03
AAACAGG255.4487544E-540.040
ACGTTGA255.4487544E-540.010
CGCCTAC407.9835445E-940.027
TTTCCGC407.9835445E-940.023
Produced by FastQC (version 0.11.2)