000000000-AKRU9_l01n01_exp163gap1bff_68.3510000004f799.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_68.3510000004f799.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186493
Sequences flagged as poor quality	0
Sequence length	46
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	17071	9.153694776747653	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	2058	1.1035266739234182	No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	719	0.38553725877110673	No Hit
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA	477	0.2557736751513462	RNA PCR Primer, Index 41 (96% over 30bp)
AACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGG	428	0.22949923053412194	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	425	0.22789059106776127	RNA PCR Primer, Index 41 (96% over 28bp)
AACATACATATCACCATTATCGAACTCAACGCCCTGCATACGAAAA	282	0.15121210983790276	No Hit
ATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCA	282	0.15121210983790276	No Hit
GTCCACGAGGTCTCTGCGACATACACACAGCGGTACATACGCTGCA	251	0.13458950201884254	No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	248	0.13298086255248187	No Hit
AAACTATCAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGTA	247	0.1324446493970283	No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA	232	0.12440145206522497	No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA	228	0.12225659944341075	No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG	219	0.11743068104432873	No Hit
GTCCACGAGGTCTCTTACATACTAAGAGAGCCGCCCGTACGCTGCA	215	0.11528582842251453	No Hit
CCTAGTTTTGTTTCTGGTGCTATGGCTAAAGCTGGTAAAGGACTTC	214	0.11474961526706097	No Hit
GTCCACGAGGTCTCTGATGACAGTACAGTTGACAGCGTACGCTGCA	213	0.11421340211160741	No Hit
TTTCCAGAAATTGTTCCAAGTATCGGCAACAGCTTTATCAATACCA	210	0.11260476264524674	No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG	210	0.11260476264524674	No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA	209	0.11206854948979318	No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG	208	0.11153233633433962	No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA	206	0.11045991002343251	No Hit
GTCCACGAGGTCTCTTGTCCGGCCAAAGAGCCAAACGTACGCTGCA	206	0.11045991002343251	No Hit
GTCCACGAGGTCTCTACCCAGTAGTGAACAGGAGCCGTACGCTGCA	205	0.10992369686797895	No Hit
GTCCACGAGGTCTCTAGATGTAGAGCATATACCGGCGTACGCTGCA	202	0.10831505740161829	No Hit
GTCCACGAGGTCTCTGGGCGTCCAACAATGCACAACGTACGCTGCA	201	0.10777884424616473	No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA	201	0.10777884424616473	No Hit
AAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAATA	199	0.10670641793525762	No Hit
GTCCACGAGGTCTCTGGCTAGAACCAATTCCCAGACGTACGCTGCA	193	0.1034891390025363	No Hit
GTCCACGAGGTCTCTACAGTATGGACAGTTCCGCGCGTACGCTGCA	191	0.10241671269162918	No Hit
GTCCACGAGGTCTCTACAGCACTGCAATGACGGTACGTACGCTGCA	189	0.10134428638072207	No Hit
TTCATTTCCATGCGGTGCACTTTATGCGGACACTTCCTACAGGTAG	187	0.10027186006981495	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	50	7.6397555E-11	40.000004	23
TCGCCTG	25	7.769523E-5	40.000004	15
CTTGTCC	25	7.769523E-5	40.000004	14
GTTTGTT	25	7.769523E-5	40.000004	24
CTTAAGA	25	7.769523E-5	40.000004	14
ATTTACC	25	7.769523E-5	40.000004	21
CTTAAAC	25	7.769523E-5	40.000004	22
AACAATG	25	7.769523E-5	40.000004	24
TCTGATT	25	7.769523E-5	40.000004	13
CCTTACA	25	7.769523E-5	40.000004	17
GCCCATA	25	7.769523E-5	40.000004	23
AAATCTA	25	7.769523E-5	40.000004	25
GGTACCG	25	7.769523E-5	40.000004	31
AAATCGC	50	7.6397555E-11	40.000004	25
CAAACAC	25	7.769523E-5	40.000004	24
AATTTGA	25	7.769523E-5	40.000004	26
TCCGCAT	50	7.6397555E-11	40.000004	15
TTAATAA	25	7.769523E-5	40.000004	24
TTCAGGA	25	7.769523E-5	40.000004	15
CAACGTG	25	7.769523E-5	40.000004	25

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content