FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_67.3510000004f78c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_67.3510000004f78c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATACCACTGACCCTCAGCAATCTTAAACTTCTTAGACGAATCA47016.057396651861975No Hit
AAGTGCACCGCATGGAAATGAAGACGGCCATTAGCTGTACCATACT41214.075845575674753No Hit
CTGTAACCATAAGGCCACGTATTTTGCAAGCTATTTAACTGGCGGC39113.358387427400068No Hit
CGAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGTTATCCATCT31710.830201571574992No Hit
AGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTC2679.121967885206695No Hit
ATCTCGGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCATAA1926.559617355654254No Hit
AATTTGAGCAGATTTGTCGTCACAGGTTGCGCCGCCAAAACGTCGG1916.525452681926888No Hit
CTGACCAAGAACGTGATTACTTCATGCAGCGTTACCATGATGTTAT1725.876323881106935No Hit
TACCTGGTCTTTCGTATTCTGGCGTGAAGTCGCCGACTGAATGCCA1495.09053638537752No Hit
CGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAA1344.578066279467031No Hit
TTAAGCCACTTCTCCTCATCCAACGCGTCAGTTTTTGACAGAATCG983.3481380252818584No Hit
AAGTTCACCGCATGGAAATGAAGACGGCCATTAGCTGTACCATACT40.1366586949094636No Hit
AAGTGCACCGCATGGAAATGAAGACGTCCATTAGCTGTACCATACT30.10249402118209772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG351.886292E-740.035
TAGAAGT303.3964552E-640.036
TGATGCG700.040.029
AAACCTG256.0365324E-540.08
AAACTTC351.886292E-740.028
TGTTGCT256.0365324E-540.016
TTTTGCA303.3964552E-640.022
GTAGAAG303.3964552E-640.035
CAAGAGC700.040.021
GTGTTTT256.0365324E-540.034
AATCTTA351.886292E-740.022
TGCACCG455.5661076E-1040.04
TTGCAGA303.3964552E-640.07
ATCTCTT700.040.013
TAAACTT351.886292E-740.027
TTGCAAG303.3964552E-640.024
AATCTCT700.040.012
ATCTCGG256.0365324E-540.01
ATCCATC700.040.039
AGCTTGC303.3964552E-640.04
Produced by FastQC (version 0.11.2)