FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_66.3510000004f77f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_66.3510000004f77f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268905
Sequences flagged as poor quality0
Sequence length46
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA5926022.03752254513676Illumina PCR Primer Index 11 (96% over 28bp)
TGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA156995.83812126959335Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC26380.9810156003049405No Hit
TGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC16860.6269872259719975Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA16570.6162027481824436RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA8650.32167494096428106No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA5540.20602071363492683RNA PCR Primer, Index 41 (96% over 28bp)
TGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACA5140.19114557185623177Illumina PCR Primer Index 11 (96% over 28bp)
TGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT4440.16511407374351536Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA4070.1513545675982224Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA3570.13276064037485358Illumina PCR Primer Index 11 (96% over 28bp)
TCAAAGTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAGAAC3170.11788549859615849No Hit
TGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3120.1160261058738216Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTACATACTAAGAGAGCCGCCCGTACGCTGCA3060.11379483460701735No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA3060.11379483460701735No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA2880.10710102080660455No Hit
GTTCTTGCAAATCACCAGAAGGCGGTTCCTGAATGAATGGGAAGCC2820.10486974953980029No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA2800.10412599245086555No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA2780.1033822353619308No Hit
GTCCACGAGGTCTCTGGGCGTCCAACAATGCACAACGTACGCTGCA2760.10263847827299605No Hit
GTCCACGAGGTCTCTACAGCACTGCAATGACGGTACGTACGCTGCA2730.10152284263959391No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA2700.10040720700619178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCC2900.040.00000415
TATCCCT2900.040.00000416
TAATCAA200.001254054540.020
TCAAAAT257.778715E-540.07
CGGACAA257.778715E-540.022
TCCGGCT257.778715E-540.015
AGCACCT257.778715E-540.017
CCCTGAA200.001254054540.017
ATTTATC257.778715E-540.028
CCTTCCG451.2132659E-940.031
GTTTGTT507.6397555E-1140.024
GTTTGTA304.8535985E-640.033
GTTGAGT353.0447336E-740.036
CTTAAGC451.2132659E-940.014
ACAACGT401.918852E-840.032
TCGCCAG401.918852E-840.015
GTGTTCA257.778715E-540.022
CTAGATT304.8535985E-640.014
CATGGTC200.001254054540.026
GTTTGGA200.001254054540.029
Produced by FastQC (version 0.11.2)