FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_65.3510000004f762.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_65.3510000004f762.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367787
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC65421.778746937765609No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA45881.2474611663816284Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA16640.45243578484285746No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA4540.12344101341265461No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA4490.12208153088608352No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA4300.1169154972851134No Hit
GACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCAC4280.11637170427448497No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA4220.11474032524259965No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA4160.11310894621071436No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG4020.10930239513631532No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA3680.10005791395563193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTT200.001254655540.00000428
CGGACAA200.001254655540.00000422
TCCGGCT304.858477E-640.00000415
TCCGGAT200.001254655540.00000415
TCCGGAC200.001254655540.00000415
CCTTCGC200.001254655540.00000428
TTGATTT304.858477E-640.00000430
ACAACTA200.001254655540.00000424
GTTTGTT600.040.00000424
ATTTAGG304.858477E-640.00000418
GTTGAGG200.001254655540.00000425
GTTGAGA304.858477E-640.00000424
GTGTTGC200.001254655540.00000430
CTTAAGA401.9221261E-840.00000414
ACAACGA200.001254655540.00000424
GCCCCCC200.001254655540.00000421
GAAATTG200.001254655540.00000424
GTTGAAA200.001254655540.00000421
CTTAAAC304.858477E-640.00000422
ATCCTAC200.001254655540.00000422
Produced by FastQC (version 0.11.2)