Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-AKRU9_l01n01_exp163gap1bff_64.3510000004f756.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 351477 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 64128 | 18.245290588004337 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC | 3786 | 1.0771686340784745 | No Hit |
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 1803 | 0.5129780896047251 | RNA PCR Primer, Index 41 (96% over 28bp) |
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA | 1236 | 0.35165885676729913 | RNA PCR Primer, Index 41 (96% over 30bp) |
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA | 1199 | 0.34113185215533304 | No Hit |
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA | 522 | 0.14851611912017004 | RNA PCR Primer, Index 41 (96% over 28bp) |
TAGAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTT | 495 | 0.1408342508898164 | No Hit |
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA | 474 | 0.13485946448843025 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA | 402 | 0.1143744825408206 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGGACAA | 30 | 4.8578604E-6 | 40.000004 | 22 |
TCCGGCT | 30 | 4.8578604E-6 | 40.000004 | 15 |
CTTGTAT | 30 | 4.8578604E-6 | 40.000004 | 14 |
CTTAAGA | 60 | 0.0 | 40.000004 | 14 |
CTAGATC | 60 | 0.0 | 40.000004 | 14 |
AACGTGC | 30 | 4.8578604E-6 | 40.000004 | 26 |
CGGAATG | 30 | 4.8578604E-6 | 40.000004 | 28 |
ATGGGAT | 30 | 4.8578604E-6 | 40.000004 | 27 |
CTAGAAC | 30 | 4.8578604E-6 | 40.000004 | 18 |
AACCGCC | 60 | 0.0 | 40.000004 | 26 |
TGATAAA | 30 | 4.8578604E-6 | 40.000004 | 27 |
AATCCGC | 30 | 4.8578604E-6 | 40.000004 | 26 |
CAAACCG | 30 | 4.8578604E-6 | 40.000004 | 31 |
CTCGTGG | 30 | 4.8578604E-6 | 40.000004 | 29 |
TTGTGTA | 30 | 4.8578604E-6 | 40.000004 | 33 |
GGTAAGT | 30 | 4.8578604E-6 | 40.000004 | 23 |
GTACAGG | 30 | 4.8578604E-6 | 40.000004 | 23 |
CTCAAAT | 30 | 4.8578604E-6 | 40.000004 | 22 |
CTCCGGA | 60 | 0.0 | 40.000004 | 14 |
CATGCTG | 30 | 4.8578604E-6 | 40.000004 | 26 |