FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_64.3510000004f756.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_64.3510000004f756.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351477
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA6412818.245290588004337Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC37861.0771686340784745No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA18030.5129780896047251RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA12360.35165885676729913RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA11990.34113185215533304No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA5220.14851611912017004RNA PCR Primer, Index 41 (96% over 28bp)
TAGAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTT4950.1408342508898164No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA4740.13485946448843025Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA4020.1143744825408206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA304.8578604E-640.00000422
TCCGGCT304.8578604E-640.00000415
CTTGTAT304.8578604E-640.00000414
CTTAAGA600.040.00000414
CTAGATC600.040.00000414
AACGTGC304.8578604E-640.00000426
CGGAATG304.8578604E-640.00000428
ATGGGAT304.8578604E-640.00000427
CTAGAAC304.8578604E-640.00000418
AACCGCC600.040.00000426
TGATAAA304.8578604E-640.00000427
AATCCGC304.8578604E-640.00000426
CAAACCG304.8578604E-640.00000431
CTCGTGG304.8578604E-640.00000429
TTGTGTA304.8578604E-640.00000433
GGTAAGT304.8578604E-640.00000423
GTACAGG304.8578604E-640.00000423
CTCAAAT304.8578604E-640.00000422
CTCCGGA600.040.00000414
CATGCTG304.8578604E-640.00000426