FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_63.3510000004f749.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_63.3510000004f749.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58316
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA16062.7539611770354617Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC5981.0254475615611496No Hit
AGCTTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACA5140.8814047602716236No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA3030.5195829617943617No Hit
GCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCTGGTATT2850.4887166472323205No Hit
AATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCG2820.48357226147198024No Hit
GGTAGAAGTCGTCATTTGGCGAGAAAGCTCAGTCTCAGGAGGAAGC2670.4578503326702792No Hit
ATCCATCTGAATGCAATGAAGAAAACCACCATTACCAGCATTAACC2610.4475615611495987No Hit
TTATCCTTTGGATGGTCGCCATGATGGTGGTTATTATACCGTCAAG2180.373825365251389No Hit
TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACAACCGT2050.3515330269565814No Hit
TGATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTT1260.21606420193428905No Hit
TTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGT1090.1869126826256945No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA990.1697647300912271No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA910.15604636806365318No Hit
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA800.13718362027573908No Hit
GTCCACGAGGTCTCTCCCGCAGACAGAATCATTTACGTACGCTGCA780.1337540297688456No Hit
GTCCACGAGGTCTCTTGGCTCACGAAACCCGGACACGTACGCTGCA770.13203923451539887No Hit
GTCCACGAGGTCTCTATAGCGAGCTTGAACACCGCCGTACGCTGCA750.12860964400850539No Hit
GTCCACGAGGTCTCTACAGCACTGCAATGACGGTACGTACGCTGCA730.1251800535016119No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA710.12175046299471844No Hit
GTCCACGAGGTCTCTCGGTAACGTATAATCCTGTCCGTACGCTGCA690.11832087248782495No Hit
GTCCACGAGGTCTCTAGATGGCTGCTCATGCTCCTCGTACGCTGCA690.11832087248782495No Hit
GTCCACGAGGTCTCTCCTAGAGGCTAATTTGCATCCGTACGCTGCA680.11660607723437823No Hit
GTCCACGAGGTCTCTGCGCACTAGTTTACGGCCTCGTACGCTGCAG680.11660607723437823No Hit
GTCCACGAGGTCTCTAGATGTAGAGCATATACCGGCGTACGCTGCA680.11660607723437823No Hit
GTCCACGAGGTCTCTCTACGTCGGCTCATAGTCGTCGTACGCTGCA670.11489128198093149No Hit
GTCCACGAGGTCTCTGCGACATACACACAGCGGTACATACGCTGCA670.11489128198093149No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA670.11489128198093149No Hit
GTCCACGAGGTCTCTAGAGGTATAACACTCACCCCGTACGCTGCAG670.11489128198093149No Hit
GTCCACGAGGTCTCTCATCTACTCGCAGGATAGGGCGTACGCTGCA660.11317648672748473No Hit
GTCCACGAGGTCTCTGCAGAAGTAAGCCCTCGACGTACGCTGCAGG660.11317648672748473No Hit
GTCCACGAGGTCTCTACGCTCGTATCACTGGTTATCGTACGCTGCA660.11317648672748473No Hit
GTCCACGAGGTCTCTCCGGTTGCCAAAGCGAGAAACGTACGCTGCA640.10974689622059126No Hit
GTCCACGAGGTCTCTTGCATGGCCTTACAGGTCTGCGTACGCTGCA640.10974689622059126No Hit
GTCCACGAGGTCTCTGCGCATAGTATATCAGTCTGCGTACGCTGCA610.10460251046025104No Hit
GTCCACGAGGTCTCTGATCGACGACCAGTTACTTCCGTACGCTGCA610.10460251046025104No Hit
GTCCACGAGGTCTCTGCCTATGTCACAATCTGTCACGTACGCTGCA600.10288771520680431No Hit
GTCCACGAGGTCTCTTAACGGAGGCGAACTTAGCCGTACGCTGCAG600.10288771520680431No Hit
GTCCACGAGGTCTCTCAGTGATACGCAGTTCGCTGCTACGCTGCAG600.10288771520680431No Hit
GTCCACGAGGTCTCTTATTGGGAGCCAGGTTTCATCGTACGCTGCA600.10288771520680431No Hit
GTCCACGAGGTCTCTACTTTACATTGGGCTAAGGGCGTACGCTGCA590.10117291995335757No Hit
GTCCACGAGGTCTCTGAGATAAGGAATGCCACCCCGTACGCTGCAG590.10117291995335757No Hit
GTCCACGAGGTCTCTCACTACGACTTCGGATACATCGTACGCTGCA590.10117291995335757No Hit
GTCCACGAGGTCTCTGCAGATAATACACTGTCACCCGTACGCTGCA590.10117291995335757No Hit
GTCCACGAGGTCTCTCGCACGATACACGTACTTTACGTACGCTGCA590.10117291995335757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT200.001245990740.00000417
ACACGTA200.001245990740.00000433
GGTGATT200.001245990740.00000411
AACGTAC800.040.00000434
GCCTTTG200.001245990740.00000429
AAACCAC200.001245990740.00000423
CTGAGTC200.001245990740.00000414
CAATGAA200.001245990740.00000414
CTGAGGG200.001245990740.00000439
CTGAGAG200.001245990740.00000414
TCTAGGC200.001245990740.00000413
TGCGCTT200.001245990740.00000415
AGAAGTT200.001245990740.00000424
GCAAGCC200.001245990740.00000425
ACATGAT200.001245990740.00000425
TTCGTCT200.001245990740.00000416
CTGGGTA200.001245990740.00000414
TTCCGTT200.001245990740.0000042
CTCCTGA200.001245990740.00000414
ACCATTA200.001245990740.00000428
Produced by FastQC (version 0.11.2)