FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_61.3510000004f72f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_61.3510000004f72f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297076
Sequences flagged as poor quality0
Sequence length46
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA4133913.915294402779088Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC30751.0350886641802097No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA13120.44163783005022283No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA10750.36186026471340665RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA8360.2814094709771237RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG6100.20533466183737495No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA5190.1747027696616354No Hit
TCGCGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAA3520.11848819830615734No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA3430.11545867050855674No Hit
ACGCCCTGCATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATGC3270.1100728433128223No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA3120.1050236303168213RNA PCR Primer, Index 41 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAAG257.780685E-540.037
CGGACAA304.8553175E-640.022
CCCTGAT257.780685E-540.017
TCCGGAT257.780685E-540.015
TCCGGAA304.8553175E-640.015
TGATCAG200.001254266640.015
CCTTCGT1000.040.032
CCTTCGC200.001254266640.028
CCTTCCG451.2150849E-940.031
TCTGCAG2000.040.013
GTTTGTT507.6397555E-1140.024
GTGTTGC200.001254266640.030
ACAACGC507.6397555E-1140.025
GTCATGA304.8553175E-640.016
TATTTAA200.001254266640.022
CTTCGGT200.001254266640.014
GTTTGGA304.8553175E-640.029
GTTGAAA304.8553175E-640.021
TTGATAG257.780685E-540.015
GTTTGCG200.001254266640.031
Produced by FastQC (version 0.11.2)