FastQC Report![[OK]](data:image/png;base64,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)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 000000000-AKRU9_l01n01_exp163gap1bff_60.3510000004f712.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 217225 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 46 |
| %GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
![[FAIL]](data:image/png;base64,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)
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 27327 | 12.580043733456096 | Illumina PCR Primer Index 11 (96% over 28bp) |
| GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC | 2224 | 1.0238232247669465 | No Hit |
| GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 755 | 0.34756588790424675 | RNA PCR Primer, Index 41 (96% over 28bp) |
| GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA | 754 | 0.34710553573483716 | No Hit |
| GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA | 726 | 0.3342156749913684 | RNA PCR Primer, Index 41 (96% over 30bp) |
| TAAAATAGTTGTTATAGATATTCAAATAACCCTGAAACAAATGCTT | 548 | 0.2522729888364599 | No Hit |
| CGGCGTTATAACCTCACACTCAATCTTTTATCACGAAGTCATGATT | 398 | 0.18322016342502015 | No Hit |
| AAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACG | 358 | 0.1648060766486362 | No Hit |
| GTCCACGAGGTCTCTGCGACATACACACAGCGGTACATACGCTGCA | 271 | 0.12475543791000115 | No Hit |
| GTCCACGAGGTCTCTTGTCCGGCCAAAGAGCCAAACGTACGCTGCA | 256 | 0.11785015536885718 | No Hit |
| TCCTGCTCCTGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGTT | 242 | 0.11140522499712278 | No Hit |
| GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA | 240 | 0.1104845206583036 | No Hit |
| GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA | 239 | 0.11002416848889401 | No Hit |
| GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA | 236 | 0.10864311198066522 | No Hit |
| GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA | 233 | 0.10726205547243642 | No Hit |
| GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA | 227 | 0.10449994245597882 | No Hit |
| GTCCACGAGGTCTCTACCCAGTAGTGAACAGGAGCCGTACGCTGCA | 227 | 0.10449994245597882 | No Hit |
| GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA | 224 | 0.10311888594775004 | No Hit |
| GTCCACGAGGTCTCTGAGTCGGCTACACTCTATGCGTACGCTGCAG | 224 | 0.10311888594775004 | No Hit |
| GTCCACGAGGTCTCTGAGATAAGGAATGCCACCCCGTACGCTGCAG | 223 | 0.10265853377834043 | No Hit |
| GTCCACGAGGTCTCTGGTATGACTATCACCCATCGCGTACGCTGCA | 218 | 0.10035677293129244 | No Hit |
Adapter Content
Kmer Content
| Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| CCTTCGT | 60 | 0.0 | 40.000004 | 32 |
| ACAACTC | 30 | 4.8492766E-6 | 40.000004 | 14 |
| GTTGAAA | 30 | 4.8492766E-6 | 40.000004 | 21 |
| ATCCTCC | 30 | 4.8492766E-6 | 40.000004 | 30 |
| GTGCACG | 30 | 4.8492766E-6 | 40.000004 | 31 |
| AGCCGCT | 30 | 4.8492766E-6 | 40.000004 | 27 |
| GTACAAA | 30 | 4.8492766E-6 | 40.000004 | 17 |
| AAATAGT | 55 | 5.456968E-12 | 40.000004 | 3 |
| AAATAAC | 55 | 5.456968E-12 | 40.000004 | 24 |
| CGAAACG | 30 | 4.8492766E-6 | 40.000004 | 31 |
| AACCCTA | 30 | 4.8492766E-6 | 40.000004 | 26 |
| ACTGAAA | 30 | 4.8492766E-6 | 40.000004 | 21 |
| GCTGGCG | 30 | 4.8492766E-6 | 40.000004 | 31 |
| TGTGGTC | 30 | 4.8492766E-6 | 40.000004 | 30 |
| CGGCACT | 30 | 4.8492766E-6 | 40.000004 | 20 |
| TCTTAGT | 110 | 0.0 | 40.000004 | 13 |
| TCTTAGG | 30 | 4.8492766E-6 | 40.000004 | 13 |
| GTCTCGG | 30 | 4.8492766E-6 | 40.000004 | 10 |
| CTCCCAG | 30 | 4.8492766E-6 | 40.000004 | 14 |
| CACGCGT | 55 | 5.456968E-12 | 40.000004 | 32 |