FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_6.3510000004f3b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_6.3510000004f3b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171927
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA22871.330215731095174Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC20191.1743356191872132No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA8070.46938526234971817No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG6800.3955167018560203No Hit
AACAAAGAAACGCGGCACAGAATGTTTATAGGTCTGTTGAACACGA4630.2693003425872609No Hit
GTTAATCGTGCCAAGAAAAGCGGCATGGTCAATATAACCAGTAGTG3090.1797274424610445No Hit
AAATGCAGCAGCAAGATAATCACGAGTATCCTTTCCTTTATCAGCG2640.15355354307351377No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2520.14657383657017223No Hit
CACACAAAAATACTGATAGCAGTCGGCGTGTGAATCATTAGCCTTG2350.1366859190237717No Hit
AGGAAGCGGAGCAGTCCAAATGTTTTTGAGATGGCAGCAACGGAAA2220.12912457031181837No Hit
TTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTT2140.12447143264292403No Hit
GTCCACGAGGTCTCTTATGCGCTGGCAGTTCTCCTCGTACGCTGCA2110.12272650601708865No Hit
GTCCACGAGGTCTCTAGACTATCTCTAATGACCCGCGTACGCTGCA2070.12039993718264148No Hit
TCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA1900.11051201963624097RNA PCR Primer, Index 41 (96% over 29bp)
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA1900.11051201963624097No Hit
GTCCACGAGGTCTCTCATGAGTCGTGATACGCTTCGTACGCTGCAG1880.10934873521901738No Hit
GTTAGGAACATTAGAGCCTTGAATGGCAGATTTAATACCAGCATCA1880.10934873521901738No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA1850.10760380859318198No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA1790.10411395534151123No Hit
CATCCACGGCGCTTTAAAATAGTTGTTATAGATATTCAAATAACCC1780.10353231313289943No Hit
GTCCACGAGGTCTCTGCAGAAGTAAGCCCTCGACGTACGCTGCAGG1780.10353231313289943No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA1750.10178738650706404No Hit
GTCCACGAGGTCTCTGGTGCTCAGTCATCTTCACTCGTACGCTGCA1730.10062410208984045No Hit
GTCCACGAGGTCTCTTATTGGGAGCCAGGTTTCATCGTACGCTGCA1730.10062410208984045No Hit
GTCCACGAGGTCTCTGGCCATAACATGCAAGTGTACGTACGCTGCA1720.10004245988122866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT7900.040.00000412
GCATAAT200.001252792540.023
TAATCAA200.001252792540.020
TCACGGT304.843365E-640.015
CGGACAA200.001252792540.022
CCTTCGC304.843365E-640.028
CTTAATC200.001252792540.018
CTTGTGG200.001252792540.014
TCTGCCC1200.040.013
ATCCTTT200.001252792540.028
TGTTTTT257.766991E-540.021
GTTTGTT257.766991E-540.024
CTTGTAT200.001252792540.014
TCGCCGT304.843365E-640.032
TATTTGC257.766991E-540.016
TCGCCCG200.001252792540.031
ACAACGT257.766991E-540.032
TCGCCAG200.001252792540.015
TTCTATA200.001252792540.015
ACAACGC200.001252792540.025
Produced by FastQC (version 0.11.2)