Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-AKRU9_l01n01_exp163gap1bff_59.3510000004f705.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 384284 |
Sequences flagged as poor quality | 0 |
Sequence length | 46 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 48767 | 12.690354009014165 | Illumina PCR Primer Index 11 (96% over 28bp) |
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA | 3954 | 1.0289265230922964 | RNA PCR Primer, Index 41 (96% over 30bp) |
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC | 3395 | 0.8834611901614432 | No Hit |
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA | 1491 | 0.38799429588533485 | No Hit |
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA | 1240 | 0.3226780193814991 | RNA PCR Primer, Index 41 (96% over 28bp) |
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG | 494 | 0.12855075933424237 | No Hit |
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG | 480 | 0.12490762040574159 | No Hit |
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA | 426 | 0.11085551311009564 | No Hit |
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA | 416 | 0.10825327101830938 | No Hit |
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA | 390 | 0.10148744157966504 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATTTATT | 30 | 4.8590464E-6 | 40.000004 | 27 |
ACAACTA | 30 | 4.8590464E-6 | 40.000004 | 24 |
CTTAAGT | 30 | 4.8590464E-6 | 40.000004 | 14 |
ATCCTGT | 30 | 4.8590464E-6 | 40.000004 | 28 |
TTAGGTC | 30 | 4.8590464E-6 | 40.000004 | 15 |
CGGAATG | 30 | 4.8590464E-6 | 40.000004 | 28 |
TAATAGG | 60 | 0.0 | 40.000004 | 15 |
AACAACG | 30 | 4.8590464E-6 | 40.000004 | 24 |
TCGTTTC | 30 | 4.8590464E-6 | 40.000004 | 31 |
TGCCCTA | 30 | 4.8590464E-6 | 40.000004 | 15 |
GCAAGTC | 30 | 4.8590464E-6 | 40.000004 | 24 |
TCGCACT | 30 | 4.8590464E-6 | 40.000004 | 23 |
GATAATA | 30 | 4.8590464E-6 | 40.000004 | 19 |
TCCGCAT | 60 | 0.0 | 40.000004 | 15 |
TTGGAGA | 30 | 4.8590464E-6 | 40.000004 | 15 |
GATCGGA | 30 | 4.8590464E-6 | 40.000004 | 19 |
CTCAACG | 30 | 4.8590464E-6 | 40.000004 | 14 |
GGAGATC | 30 | 4.8590464E-6 | 40.000004 | 30 |
TCATTTG | 30 | 4.8590464E-6 | 40.000004 | 26 |
CTTCCAT | 60 | 0.0 | 40.000004 | 14 |