FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_59.3510000004f705.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_59.3510000004f705.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384284
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA4876712.690354009014165Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA39541.0289265230922964RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC33950.8834611901614432No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA14910.38799429588533485No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA12400.3226780193814991RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG4940.12855075933424237No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG4800.12490762040574159No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA4260.11085551311009564No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA4160.10825327101830938No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3900.10148744157966504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTATT304.8590464E-640.00000427
ACAACTA304.8590464E-640.00000424
CTTAAGT304.8590464E-640.00000414
ATCCTGT304.8590464E-640.00000428
TTAGGTC304.8590464E-640.00000415
CGGAATG304.8590464E-640.00000428
TAATAGG600.040.00000415
AACAACG304.8590464E-640.00000424
TCGTTTC304.8590464E-640.00000431
TGCCCTA304.8590464E-640.00000415
GCAAGTC304.8590464E-640.00000424
TCGCACT304.8590464E-640.00000423
GATAATA304.8590464E-640.00000419
TCCGCAT600.040.00000415
TTGGAGA304.8590464E-640.00000415
GATCGGA304.8590464E-640.00000419
CTCAACG304.8590464E-640.00000414
GGAGATC304.8590464E-640.00000430
TCATTTG304.8590464E-640.00000426
CTTCCAT600.040.00000414