FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_58.3510000004f6f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_58.3510000004f6f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222628
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA165047.413263381066174Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC27361.2289559264782506No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA10430.4684945289900641No Hit
TTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCC4600.2066227069371328No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA4350.19539321199489734RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA3950.17742602008732056No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA3750.16844242413353216No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG3410.15317031101209191No Hit
GTCCACGAGGTCTCTTGTCCGGCCAAAGAGCCAAACGTACGCTGCA3330.14957687263057656No Hit
ATAAACGTGACGATGAGGGACATAAAAAGTAAAAATGTCTACAGTA3080.1383473776883411No Hit
TCACTTAAGTGGCTGGAGACAAATAATCTCTTTAATAACCTGATTC2980.1338555797114469No Hit
GTCCACGAGGTCTCTGGGCGTCCAACAATGCACAACGTACGCTGCA2970.1334063999137575No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2870.1289146019368633No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG2850.12801624234148445No Hit
GTCCACGAGGTCTCTTACATACTAAGAGAGCCGCCCGTACGCTGCA2840.12756706254379505No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA2830.1271178827461056No Hit
GTCCACGAGGTCTCTGGTATGACTATCACCCATCGCGTACGCTGCA2820.1266687029484162No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA2790.12532116355534795No Hit
GTCCACGAGGTCTCTTAGGTCATCACACGCAGTCCGTACGCTGCAG2760.12397362416227968No Hit
GTCCACGAGGTCTCTTCATGGCGTTAATGGCTATGCGTACGCTGCA2730.12262608476921143No Hit
GTCCACGAGGTCTCTGATGACAGTACAGTTGACAGCGTACGCTGCA2690.12082936557845374No Hit
GTCCACGAGGTCTCTACAGCACTGCAATGACGGTACGTACGCTGCA2600.11678674739924898No Hit
AGAACGGAAAACATCCTTCATAGAAATTTCACGCGGCGGCAAGTTG2600.11678674739924898No Hit
GTCCACGAGGTCTCTGAGTCACACGCACCATATAGCGTACGCTGCA2570.11543920800618072No Hit
GTCCACGAGGTCTCTGCCATATACTAAGACCTTGCCGTACGCTGCA2560.11499002820849129No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA2540.11409166861311246No Hit
GTCCACGAGGTCTCTAACTCATGTGAATGCAGGGCCGTACGCTGCA2530.11364248881542303No Hit
GTCCACGAGGTCTCTGCGGCTGCAATACAGTTCTACGTACGCTGCA2450.11004905043390768No Hit
GTCCACGAGGTCTCTTCTGACAGCAAATACGCTCACGTACGCTGCA2440.10959987063621826No Hit
GTCCACGAGGTCTCTATGTACGCCTGCACTTGGCTCGTACGCTGCA2390.10735397164777116No Hit
GTCCACGAGGTCTCTGCGCCACAGCACTTAATATACGTACGCTGCA2330.10465889286163466No Hit
GTCCACGAGGTCTCTGCCATTATAGCAATCTGCACCGTACGCTGCA2230.10016709488474046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTC200.001253588840.00000415
TTTAGTA200.001253588840.00000428
ATTTAGT200.001253588840.00000427
CTTAAGA200.001253588840.00000414
TCGCCCC200.001253588840.00000430
TTCTATG401.9163053E-840.00000415
GTTTGGC200.001253588840.00000423
ATGGGTA200.001253588840.00000422
TGTTTCC200.001253588840.00000430
CATGGGT200.001253588840.00000421
GTGCAAC200.001253588840.00000422
GAAATCA200.001253588840.00000424
CTAGAAT200.001253588840.00000414
TGTCAAG200.001253588840.00000422
CTAGAAC200.001253588840.00000418
GAAATAC200.001253588840.00000430
TAATAGG200.001253588840.00000415
TAATAGA200.001253588840.00000415
GATACTA200.001253588840.00000418
ACTATAT200.001253588840.00000426
Produced by FastQC (version 0.11.2)