000000000-AKRU9_l01n01_exp163gap1bff_57.3510000004f6ea.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_57.3510000004f6ea.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1807
Sequences flagged as poor quality	0
Sequence length	46
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTTCCAGAAATTGTTCCAAGTATCGGCAACAGCTTTATCAATACC	271	14.997232982844494	No Hit
CAAACTATCAAAATATAACGTTGACGATGTAGCTTTAGGTGTCTGT	246	13.613724405091313	No Hit
CTTCTCGTTCTCTAAAAACCATTTTTCGTCCCCTTCGGGGCGGTGG	218	12.064194798007748	No Hit
CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAAT	187	10.348644161593802	No Hit
CAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCT	151	8.35639180962922	No Hit
CCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATG	122	6.751521859435529	No Hit
TTATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGA	108	5.976757055893747	No Hit
ACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATG	106	5.866076369673491	No Hit
CCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	24	1.3281682346430548	Illumina PCR Primer Index 11 (96% over 28bp)
CAAACTATCAAAATATAACGTTGACGCTGTAGCTTTAGGTGTCTGT	3	0.16602102933038185	No Hit
CCACGAGGTCTCTGCCGTAGTAACAAGCTCACACGTACGCTGCAGG	3	0.16602102933038185	No Hit
CCACGAGGTCTCTTTGCACAACCGGAAATGCCATGTACGCTGCAGG	3	0.16602102933038185	No Hit
CACGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCT	2	0.11068068622025456	No Hit
CCACGAGGTCTCTTGGTAGACCCACAAACTACCGTACGCTGCAGGT	2	0.11068068622025456	No Hit
CCACGAGGTCTCTTTCATCGCGCTCATGTGGTGCGTACGCTGCAGG	2	0.11068068622025456	No Hit
CTTTCCAGACATTGTTCCAAGTATCGGCAACAGCTTTATCAATACC	2	0.11068068622025456	No Hit
CCACGAGGTCTCTAACTGTGTGCAATGGCTGCCCGTACGCTGCAGG	2	0.11068068622025456	No Hit
CCACGAGGTCTCTAAGTCGTGGTCATCTCGCTTCGTACGCTGCAGG	2	0.11068068622025456	No Hit
CCACGAGGTCTCTGTGCGCCCAAATCAGAAACGTACGCTGCAGGTC	2	0.11068068622025456	No Hit
CTTCCCAGAAATTGTTCCAAGTATCGGCAACAGCTTTATCAATACC	2	0.11068068622025456	No Hit
CCACGAGGTCTCTGCAGTCGATAGACCCGATTCCGTACGCTGCAGG	2	0.11068068622025456	No Hit
CCACGAGGTCTCTGAGGAGACTGTACTATCATGCGTACGCTGCAGG	2	0.11068068622025456	No Hit
CCACGAGGTCTCTTGTGGAACCAAAGCGCCCAACGTACGCTGCAGG	2	0.11068068622025456	No Hit
CCACGAGGTCTCTTTCGACGCGAGACTCTAGTGCGTACGCTGCAGG	2	0.11068068622025456	No Hit
CAAGAACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGGCAAT	2	0.11068068622025456	No Hit
CCACGAGGTCTCTGCGGGATGAAACAGCTACACGTACGCTGCAGGT	2	0.11068068622025456	No Hit
CCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGGTC	2	0.11068068622025456	No Hit
CCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCAGG	2	0.11068068622025456	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTCCA	20	9.507581E-4	40.0	1
GTATGTT	25	5.1145656E-5	40.0	23
TGTGACT	20	9.507581E-4	40.0	10
GAGGTCT	45	3.274181E-10	40.0	5
AAGTATC	20	9.507581E-4	40.0	19
ACTGTGT	20	9.507581E-4	40.0	6
CCAATTG	25	5.1145656E-5	40.0	17
TTGTTCC	20	9.507581E-4	40.0	12
TCCTTCC	20	9.507581E-4	40.0	24
TTCATGC	25	5.1145656E-5	40.0	30
AAACCAT	30	2.6825219E-6	40.0	16
ACGCCGG	20	9.507581E-4	40.0	35
TCCCAAT	25	5.1145656E-5	40.0	15
GCCTCCA	25	5.1145656E-5	40.0	35
CTTTATC	20	9.507581E-4	40.0	34
GAAATTG	20	9.507581E-4	40.0	8
GGGCAAT	20	9.507581E-4	40.0	40
TTGTATG	25	5.1145656E-5	40.0	21
AAATTGT	20	9.507581E-4	40.0	9
AGGACTG	20	9.507581E-4	40.0	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content