FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_56.3510000004f6dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_56.3510000004f6dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352870
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA269537.638223708447871Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC45721.2956612916938248No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA15020.42565250658882875No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA6860.19440587185082325RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG4810.1363108226825743No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA4320.1224246889789441No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA4220.11959078414146852No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG3620.10258735511661518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG1450.040.00000431
TCTTAAG1450.040.00000413
CTGACGT1450.040.00000432
GTGACTG5800.040.00000424
TCACGGT304.857917E-640.015
TGATCGC257.783664E-540.015
CGGACAA451.2169039E-940.022
TCCGGAT200.001254586740.015
TCCGGAA257.783664E-540.015
CCTTCGT1100.040.032
ATTTATT257.783664E-540.027
CTTAATG200.001254586740.014
TTGATTT200.001254586740.030
CCTTCCG600.040.031
CCTTCCC200.001254586740.022
GTTTGTT451.2169039E-940.024
ATTTAGG200.001254586740.018
CTTAAGT257.783664E-540.014
GTTGAGG257.783664E-540.025
CTTAAGA600.040.014
Produced by FastQC (version 0.11.2)