000000000-AKRU9_l01n01_exp163gap1bff_55.3510000004f6c0.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_55.3510000004f6c0.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	113382
Sequences flagged as poor quality	0
Sequence length	46
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	28407	25.054241413981053	Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA	1130	0.996630858513697	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	811	0.7152810851810693	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	795	0.7011694978038843	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	524	0.4621544866028117	RNA PCR Primer, Index 41 (96% over 30bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC	389	0.3430879681078125	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	353	0.3113368965091461	No Hit
GGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAA	259	0.22843132066818367	No Hit
AGAGTAAACATAGTGCCATGCTCAGGAACAAAGAAACGCGGCACAG	257	0.22666737224603553	No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA	241	0.2125557848688504	Illumina PCR Primer Index 11 (96% over 28bp)
TAACGATACCACTGACCCTCAGCAATCTTAAACTTCTTAGACGAAT	225	0.19844419749166534	No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA	222	0.19579827485844314	RNA PCR Primer, Index 41 (96% over 28bp)
TGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCC	207	0.18256866169233213	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA	202	0.17815879063696177	Illumina PCR Primer Index 11 (96% over 28bp)
TAAATTTGAGCAGATTTGTCGTCACAGGTTGCGCCGCCAAAACGTC	179	0.1578733837822582	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC	178	0.15699140957118413	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA	152	0.13406008008325837	RNA PCR Primer, Index 41 (96% over 30bp)
ACCTGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAA	146	0.12876823481681396	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGCAAAAAA	136	0.11994849270607327	Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC	135	0.1190665184949992	Illumina PCR Primer Index 11 (96% over 28bp)
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT	135	0.1190665184949992	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTCGATCCACAGTAGCTGAATCCGTACGCTGCA	133	0.11730257007285107	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	130	0.11465664743962886	No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	127	0.11201072480640667	No Hit
TAATCTCGGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCCAT	123	0.1084828279621104	No Hit
GGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG	121	0.10671887953996224	Illumina PCR Primer Index 11 (96% over 28bp)
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA	119	0.10495493111781411	RNA PCR Primer, Index 41 (96% over 30bp)
GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGAT	114	0.10054506006244378	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGAGC	40	1.9021172E-8	40.000004	5
CTTCAGG	20	0.0012509867	40.000004	14
AATCTTT	20	0.0012509867	40.000004	26
AACAAGC	20	0.0012509867	40.000004	24
TAAATTT	40	1.9021172E-8	40.000004	1
TTGCAAA	20	0.0012509867	40.000004	37
CATTGAT	20	0.0012509867	40.000004	26
GCCTTCG	20	0.0012509867	40.000004	31
CTATAGC	40	1.9021172E-8	40.000004	14
TAAACAT	40	1.9021172E-8	40.000004	5
ATCTTAA	20	0.0012509867	40.000004	25
CTTTAGT	20	0.0012509867	40.000004	14
AAATTTG	40	1.9021172E-8	40.000004	2
GCTAGTA	20	0.0012509867	40.000004	19
CTCCTTG	20	0.0012509867	40.000004	14
TGCGCCG	40	1.9021172E-8	40.000004	30
ACTGACC	20	0.0012509867	40.000004	11
TGACCCT	20	0.0012509867	40.000004	13
ACCACTG	20	0.0012509867	40.000004	8
CTCCTCG	20	0.0012509867	40.000004	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content