FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_54.3510000004f6b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_54.3510000004f6b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288442
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC30241.048391011017813No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA12980.4500038135916406Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA12450.4316292356868972No Hit
ATCAGGGTTAATCGTGCCAAGAAAAGCGGCATGGTCAATATAACCA4140.14352972174648632No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3970.13763598921100256No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3460.11995479160455136No Hit
GGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTG3430.11891472115711305No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG3310.11475443936735982No Hit
CAGTATGCAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAAT3230.111980918174191No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG3180.11024746742846049No Hit
GTCCACGAGGTCTCTTCGACAGCAAGACGATCTGACGTACGCTGCA3140.10886070683187607No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA3140.10886070683187607No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG3020.10470042504212285No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA2980.10331366444553845No Hit
GTCCACGAGGTCTCTGCGACATACACACAGCGGTACATACGCTGCA2910.10088683340151573No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2900.10054014325236962No Hit
GTCCACGAGGTCTCTTGTCCGGCCAAAGAGCCAAACGTACGCTGCA2900.10054014325236962No Hit
GCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGT2900.10054014325236962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAGT2150.040.00000413
TAATCAC401.9197614E-840.015
AGGTCAA200.001254205540.021
CGGACAA200.001254205540.022
CCTTCGT1250.040.032
TCGCCTC257.780122E-540.028
GTTGATG304.8548245E-640.09
CCTTCCG750.040.031
CCTTCCA257.780122E-540.023
CCTTCAA200.001254205540.022
GTTTGTT304.8548245E-640.024
GTTTGTA200.001254205540.033
CTTGTAT200.001254205540.014
TTTAGTA200.001254205540.028
TCGCCGT1050.040.032
TCGCCGC200.001254205540.015
ATTTAGT200.001254205540.027
CTGCTGT555.456968E-1240.014
CTTAAGA451.2150849E-940.014
TCGCCCC200.001254205540.030
Produced by FastQC (version 0.11.2)