000000000-AKRU9_l01n01_exp163gap1bff_53.3510000004f6a7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_53.3510000004f6a7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88718
Sequences flagged as poor quality	0
Sequence length	46
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	1808	2.0379178971572847	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	1068	1.2038143330552988	No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	445	0.5015893054397078	No Hit
AGAAGCAATACCGCCAGCAATAGCACCAAACATAAATCACCTCACT	401	0.4519939583849952	No Hit
TTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCA	338	0.3809824387384747	No Hit
GCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACC	337	0.37985527175995853	No Hit
CTTGAATGGCAGATTTAATACCAGCATCACCCATGCCTACAGTATT	321	0.3618206001036994	No Hit
TTGATGCGGTTATCCATCTGCTTATGGAAGCCAAGCATTGGGGATT	299	0.33702292657634303	No Hit
CAATACCATCAGCTTTACCGTCTTTCCAGAAATTGTTCCAAGTATC	261	0.2941905813927275	No Hit
CTCGATACGCTCAAAGTCAAAATAATCAGCGTGACATTCAGAAGGG	237	0.2671385739083388	No Hit
GGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAATCGAAATC	206	0.23219639757433666	No Hit
GTGGATTACTATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA	195	0.21979756081065852	No Hit
GCCAACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTA	156	0.1758380486485268	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	152	0.171329380734462	No Hit
CATACCGCTGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGC	146	0.1645663788633648	No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA	129	0.14540454022858945	No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG	111	0.1251155346152979	No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA	103	0.11609819878716833	No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG	99	0.11158953087310355	No Hit
TTCTCGAGCTGCGCAAGGATAGGTCGAATTTTCTCATTTTCCGCCA	93	0.10482652900200636	No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	93	0.10482652900200636	No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA	92	0.10369936202349016	No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA	91	0.10257219504497396	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCATT	25	7.736558E-5	40.000004	24
GAGGCGT	25	7.736558E-5	40.000004	32
AAACTTT	25	7.736558E-5	40.000004	16
CGTCAGA	25	7.736558E-5	40.000004	29
TTGTCGG	25	7.736558E-5	40.000004	29
TTGTCCG	25	7.736558E-5	40.000004	31
ACTCGTC	25	7.736558E-5	40.000004	26
TTGTTAC	25	7.736558E-5	40.000004	21
ACGCCGT	25	7.736558E-5	40.000004	32
CTATAGT	50	7.6397555E-11	40.000004	14
TCTAGAG	100	0.0	40.000004	13
AAATCAC	25	7.736558E-5	40.000004	34
CCAAATG	25	7.736558E-5	40.000004	23
CTCCTAG	25	7.736558E-5	40.000004	14
AACTGGA	25	7.736558E-5	40.000004	13
CCAAACT	25	7.736558E-5	40.000004	14
TCTGGGC	25	7.736558E-5	40.000004	13
GTTCCCG	25	7.736558E-5	40.000004	31
GAAAATC	25	7.736558E-5	40.000004	34
CCGCTGC	25	7.736558E-5	40.000004	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content