FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_52.3510000004f69a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_52.3510000004f69a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271780
Sequences flagged as poor quality0
Sequence length46
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA39541.454853190080212Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC32391.1917727573772905No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA13260.48789462064905437No Hit
GTCCACGAGGTCTCTGACGATATGATAACCTCTGCCGTACGCTGCA4660.17146221208330267No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA3980.1464419751269409No Hit
GTCCACGAGGTCTCTACAGCACTGCAATGACGGTACGTACGCTGCA3970.14607403046581793No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG3680.1354036352932519No Hit
GTCCACGAGGTCTCTACAGTATGGACAGTTCCGCGCGTACGCTGCA3650.134299801309883No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG3650.134299801309883No Hit
GTCCACGAGGTCTCTTGTCCGGCCAAAGAGCCAAACGTACGCTGCA3590.1320921333431452No Hit
GTCCACGAGGTCTCTGCGACATACACACAGCGGTACATACGCTGCA3580.13172418868202224No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA3510.12914857605416144No Hit
GTCCACGAGGTCTCTGCCATATACTAAGACCTTGCCGTACGCTGCA3320.12215762749282508No Hit
GTCCACGAGGTCTCTACCCAGTAGTGAACAGGAGCCGTACGCTGCA3260.11994995952608728No Hit
GTCCACGAGGTCTCTGGGCGTCCAACAATGCACAACGTACGCTGCA3220.11847818088159541No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA3160.11627051291485761No Hit
GTCCACGAGGTCTCTTAGGTCATCACACGCAGTCCGTACGCTGCAG3030.11148723232025903No Hit
CCATAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGCGAGAAAG2900.10670395172566047No Hit
GTCCACGAGGTCTCTGGCTAGAACCAATTCCCAGACGTACGCTGCA2900.10670395172566047No Hit
GTCCACGAGGTCTCTCATCTCGTAGGATCTGGCTCGTACGCTGCAG2880.10596806240341453No Hit
GTCCACGAGGTCTCTTACATACTAAGAGAGCCGCCCGTACGCTGCA2870.10560011774229155No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA2770.10192067113106189No Hit
GTCCACGAGGTCTCTTCGCGGACCAGATTACTTAGCGTACGCTGCA2770.10192067113106189No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG2750.10118478180881595No Hit
GTCCACGAGGTCTCTGATGACAGTACAGTTGACAGCGTACGCTGCA2740.10081683714769298No Hit
GTCCACGAGGTCTCTATCTGCGACGGAGTCACGATTGTACGCTGCA2730.10044889248657003No Hit
GTCCACGAGGTCTCTGTGGCACACACATATTCACACGTACGCTGCA2730.10044889248657003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT401.9190338E-840.00000415
ATTTATT200.001254077540.00000427
ATTTATC200.001254077540.00000417
ATTTATA401.9190338E-840.00000424
CTTGTGG401.9190338E-840.00000414
CCTTCCG401.9190338E-840.00000431
AGCGTTT200.001254077540.00000416
CGTGGTG200.001254077540.00000428
GAGGATG200.001254077540.00000423
CAGAATG200.001254077540.00000426
CAGGTCC200.001254077540.00000430
GAGTGTG200.001254077540.00000428
AGGTATA200.001254077540.00000417
GAAATGA200.001254077540.00000424
GAAATAG200.001254077540.00000424
AAGTACA200.001254077540.00000421
AACAAGC200.001254077540.00000424
CGTGGAC200.001254077540.00000430
GAGGAAT200.001254077540.00000429
CAGAAAT200.001254077540.00000422
Produced by FastQC (version 0.11.2)