FastQCFastQC Report
Mon 28 Mar 2016
000000000-AKRU9_l01n01_exp163gap1bff_51.3510000004f68d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKRU9_l01n01_exp163gap1bff_51.3510000004f68d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141568
Sequences flagged as poor quality0
Sequence length46
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA51063.606747287522604Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC14411.017885397830018No Hit
ACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCG7010.4951683996383363No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA6330.4471349457504521No Hit
ATTCTGATTCTGAACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTT2950.20838042495479203No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA2890.20414217902350812No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG2250.15893422242314648No Hit
TAATGTTCCTAACCCTGATGAGGCCGTCCCTAGTTTTGTTTCTGGT2140.15116410488245932No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG2110.14904498191681737No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG1890.13350474683544303No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA1660.11725813743218806No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA1570.1109007685352622No Hit
TTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATC1550.10948801989150092No Hit
GTCCACGAGGTCTCTGGCCCGCACACAATTAGGAACGTACGCTGCA1550.10948801989150092No Hit
GTCCACGAGGTCTCTAATGCTAACGCACCAGAGGCCGTACGCTGCA1440.10171790235081375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC200.001252042640.00000425
TTGATAG200.001252042640.00000415
GTCATCT200.001252042640.00000424
GTTTGCG200.001252042640.00000431
ATCCTAT200.001252042640.00000428
GTTTGAA200.001252042640.00000419
CTTCGAG401.907756E-840.00000414
CAGAATA200.001252042640.00000423
TGGAGTC200.001252042640.00000415
GGCAGTC200.001252042640.00000416
GGAATAG200.001252042640.00000424
TCTTGTG200.001252042640.00000413
AACCGCC200.001252042640.00000426
GACAGCC200.001252042640.00000430
AATCCAT200.001252042640.00000425
TCTTGCT200.001252042640.00000413
AAATCGG200.001252042640.00000425
AAATCGC200.001252042640.00000425
AAATCCT200.001252042640.00000416
CGAACGC200.001252042640.00000436
Produced by FastQC (version 0.11.2)