000000000-AKRU9_l01n01_exp163gap1bff_50.3510000004f670.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_50.3510000004f670.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156585
Sequences flagged as poor quality	0
Sequence length	46
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	13038	8.32646805249545	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	2081	1.328990644059137	No Hit
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	671	0.42852125043905864	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	291	0.1858415557045694	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	247	0.15774180157741802	No Hit
GTCCACGAGGTCTCTAGCTGGCATCGACTCTATCTCGTACGCTGCA	230	0.1468850783919277	No Hit
GTCCACGAGGTCTCTTACATACTAAGAGAGCCGCCCGTACGCTGCA	228	0.14560781684069354	No Hit
GTCCACGAGGTCTCTACTAGCGCAGTGAGGTATCGTACGCTGCAGG	227	0.14496918606507647	No Hit
GTCCACGAGGTCTCTCGAGTAAGTAGAGCTGATTCCGTACGCTGCA	225	0.14369192451384233	No Hit
GTCCACGAGGTCTCTGCGATCAGAACAGATGCCGACGTACGCTGCA	222	0.14177603218699109	No Hit
GTCCACGAGGTCTCTCTTGACAGGAGTGATAAACCGTACGCTGCAG	217	0.1385828783089057	No Hit
ATCAAAAGCAATATCAGCACCAACAGAAACAACCTGATTAGCGGCG	216	0.13794424753328863	No Hit
GTCCACGAGGTCTCTTGTGTGTCAACAACCACCAACGTACGCTGCA	216	0.13794424753328863	No Hit
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA	210	0.13411246287958617	No Hit
GTCCACGAGGTCTCTGGTATGACTATCACCCATCGCGTACGCTGCA	204	0.1302806782258837	No Hit
GTCCACGAGGTCTCTTGTCCGGCCAAAGAGCCAAACGTACGCTGCA	204	0.1302806782258837	No Hit
GTTATAACCTCACACTCAATCTTTTATCACGAAGTCATGATTGAAT	201	0.12836478589903247	No Hit
GTCCACGAGGTCTCTACCCAGTAGTGAACAGGAGCCGTACGCTGCA	196	0.12517163202094708	No Hit
GTCCACGAGGTCTCTACAGTATGGACAGTTCCGCGCGTACGCTGCA	195	0.12453300124533001	No Hit
GTCCACGAGGTCTCTAGTCGGATCACCCGCTGAATCGTACGCTGCA	194	0.12389437046971294	No Hit
GTCCACGAGGTCTCTACGTATCCTCAGTCTTGATCGTACGCTGCAG	193	0.12325573969409587	No Hit
GTCCACGAGGTCTCTAGACGGACTCGCATTCACTGCGTACGCTGCA	192	0.1226171089184788	No Hit
GTCCACGAGGTCTCTGAGTCACACGCACCATATAGCGTACGCTGCA	186	0.11878532426477632	No Hit
GTCCACGAGGTCTCTCATCTCGTAGGATCTGGCTCGTACGCTGCAG	182	0.116230801162308	No Hit
GTCCACGAGGTCTCTGTGGCACACACATATTCACACGTACGCTGCA	179	0.11431490883545678	No Hit
GCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGA	178	0.11367627805983971	No Hit
AATCTCTTTCTGATTGTCCAGTTGCATTTTAGTAAGCTCTTTTTGA	177	0.11303764728422264	No Hit
GTCCACGAGGTCTCTGATGACAGTACAGTTGACAGCGTACGCTGCA	176	0.11239901650860555	No Hit
AGATTGCGATAAACGGTCACATTAAATTTAACCTGACTATTCCACT	175	0.11176038573298848	No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA	173	0.11048312418175431	No Hit
AGCAAGAACCATACGACCAATATCACGAAAATAGTCACGCAAAGCA	172	0.10984449340613724	No Hit
GTCCACGAGGTCTCTGAATATGCGCTCCCACTGTGCGTACGCTGCA	171	0.10920586263052016	No Hit
GTCCACGAGGTCTCTTAGGTCATCACACGCAGTCCGTACGCTGCAG	171	0.10920586263052016	No Hit
AACAGCTTTATCAATACCATGAAAAATATCAACCACACCAGAAGCA	167	0.10665133952805186	No Hit
GTCCACGAGGTCTCTGCGGCTGCAATACAGTTCTACGTACGCTGCA	165	0.10537407797681769	No Hit
GTCCACGAGGTCTCTATGGTGCATGACCGGATATTCGTACGCTGCA	164	0.10473544720120062	No Hit
GTCCACGAGGTCTCTGGTCACTATACACCTACTAGTGTACGCTGCA	164	0.10473544720120062	No Hit
GTCCACGAGGTCTCTAGTATCTCACTACGGCTCATCGTACGCTGCA	163	0.10409681642558355	No Hit
GTCCACGAGGTCTCTAGACTCTGAGCCATTGTCGGCGTACGCTGCA	159	0.10154229332311525	No Hit
GTCCACGAGGTCTCTATTATAGTGCCAGACGTACGCTGCAGGTCGA	159	0.10154229332311525	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	30	4.8405855E-6	40.000004	23
TCTGCCT	60	0.0	40.000004	13
CCTTCCG	30	4.8405855E-6	40.000004	31
GTTGAAA	30	4.8405855E-6	40.000004	21
GTTTGCG	30	4.8405855E-6	40.000004	31
AGACTCA	30	4.8405855E-6	40.000004	21
AGCCGCG	30	4.8405855E-6	40.000004	31
TCTGACT	120	0.0	40.000004	13
TCTTGCG	30	4.8405855E-6	40.000004	13
ACTGCTC	30	4.8405855E-6	40.000004	17
CAAACCG	30	4.8405855E-6	40.000004	31
AATTTGA	30	4.8405855E-6	40.000004	26
TTGTGGA	30	4.8405855E-6	40.000004	15
CTCCGAT	30	4.8405855E-6	40.000004	14
CTTCCGG	30	4.8405855E-6	40.000004	19
CGACGAA	30	4.8405855E-6	40.000004	20
ACGCAAG	30	4.8405855E-6	40.000004	22
TTTGTTC	30	4.8405855E-6	40.000004	25
CTTTTCA	30	4.8405855E-6	40.000004	14
CTTCAGG	30	4.8405855E-6	40.000004	14

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content