000000000-AKRU9_l01n01_exp163gap1bff_5.3510000004f3aa.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_5.3510000004f3aa.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268647
Sequences flagged as poor quality	0
Sequence length	46
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	47879	17.822272349961104	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	2702	1.0057808201841079	No Hit
GTCCACGAGGTCGACACCTCGTATGCCGTCTTCTGCTTGAAAAAAA	1209	0.4500329428581	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	1137	0.423231973556377	No Hit
GTCCACGAGGGAAGGGAATTTGGGTGTGGCTGCAGGTCGACATCTC	724	0.2694986357562154	No Hit
GTCCACGAGGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAA	377	0.14033285314930002	RNA PCR Primer, Index 41 (96% over 28bp)
GTCCACGCTGCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAA	318	0.11837094774927694	RNA PCR Primer, Index 41 (96% over 30bp)
GTGCACTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATT	315	0.11725424069503848	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAACA	314	0.11688200501029232	Illumina PCR Primer Index 11 (96% over 28bp)
GACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTCGTCGGGTA	302	0.11241517679333847	No Hit
GTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	281	0.10459822741366923	RNA PCR Primer, Index 41 (96% over 30bp)
GTCCACGAGGTCTCTAGTACAGACTCACCCAGTAGCGTACGCTGCA	276	0.10273704898993846	No Hit
CGCTTCGATAAAAATGATTGGCGTATCCAACCTGCAGAGTTTTATC	273	0.1016203419357	No Hit
GTCCACGAGGTCTCTGACTAGGACCGATTGCACTTCGTACGCTGCA	273	0.1016203419357	No Hit
GTCCACGAGGTCTCTTATTGGGAGCCAGGTTTCATCGTACGCTGCA	271	0.1008758705662077	No Hit
GCATACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCA	271	0.1008758705662077	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCT	30	4.853575E-6	40.000004	20
GTTTGCG	30	4.853575E-6	40.000004	31
TCTGAAA	30	4.853575E-6	40.000004	13
GCAAGGC	30	4.853575E-6	40.000004	24
GGAGCCA	30	4.853575E-6	40.000004	21
AATTTTG	30	4.853575E-6	40.000004	29
TTAATGA	30	4.853575E-6	40.000004	24
ATAGGTT	30	4.853575E-6	40.000004	27
TTGTGGA	30	4.853575E-6	40.000004	15
CAAGTGG	30	4.853575E-6	40.000004	25
CTCCGGG	30	4.853575E-6	40.000004	14
CAAAAAA	30	4.853575E-6	40.000004	40
AACCCCG	30	4.853575E-6	40.000004	31
CCAGGAT	30	4.853575E-6	40.000004	18
CTTTTCA	60	0.0	40.000004	14
ATAATAT	30	4.853575E-6	40.000004	25
TAGGTCA	30	4.853575E-6	40.000004	16
GAGTAGA	30	4.853575E-6	40.000004	21
GACAACC	30	4.853575E-6	40.000004	30
ACCTGTC	30	4.853575E-6	40.000004	29