000000000-AKRU9_l01n01_exp163gap1bff_49.3510000004f663.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-AKRU9_l01n01_exp163gap1bff_49.3510000004f663.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240205
Sequences flagged as poor quality	0
Sequence length	46
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCACGAGGTCTCTTATCCCTAGTGACTGTCGTACGCTGCAGGTC	3697	1.5391020170271226	No Hit
GTCCACGAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	980	0.40798484627713827	Illumina PCR Primer Index 11 (96% over 28bp)
GTCCACGAGGTCTCTGCGACTATCGAACCATATACCGTACGCTGCA	963	0.40090755812743284	No Hit
CATCACCTTGAATGCCACCGGAGGCGGCTTTTTGACCGCCTCCAAA	653	0.2718511271622156	No Hit
GTCCACGAGGTCTCTCCGCATACGCAATAAGTGGACGTACGCTGCA	403	0.16777336025478237	No Hit
CCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAA	348	0.14487625153514705	No Hit
GCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCATTT	346	0.1440436293998876	No Hit
ATACCGCCAGCAATAGCACCAAACATAAATCACCTCACTTAAGTGG	345	0.14362731833225786	No Hit
GGAGAAACATACGAAGGCGCATAACGATACCACTGACCCTCAGCAA	337	0.14029682979122	No Hit
ATATCAGCACCAACAGAAACAACCTGATTAGCGGCGTTGACAGATG	326	0.13571740804729293	No Hit
TTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCTCAATAGCAGG	319	0.13280323057388482	No Hit
ACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCAA	310	0.1290564309652172	No Hit
ACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAG	307	0.12780749776232803	No Hit
GAAAACCTACCGCGCTTCGCTTGGTCAACCCCTCAGCGGCAAAAAT	301	0.1253096313565496	No Hit
ATGACGGCAGCAATAAACTCAACAGGAGCAGGAAAGCGAGGGTATC	283	0.11781603213921442	No Hit
GTCCACGAGGTCTCTCCTAGCTGATGACTGTACTTGTACGCTGCAG	281	0.11698341000395496	No Hit
GAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTA	279	0.11615078786869548	No Hit
GATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGT	278	0.11573447680106574	No Hit
GTCCACGAGGTCTCTCTACGTCGGCTCATAGTCGTCGTACGCTGCA	274	0.11406923253054684	No Hit
GTCCACGAGGTCTCTCAGACGAGCTAATTCTATCCCGTACGCTGCA	264	0.1099061218542495	No Hit
GGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTG	262	0.10907349971899002	No Hit
GTCCACGAGGTCTCTGATAGATCCATCACGCTCCGTACGCTGCAGG	260	0.10824087758373056	No Hit
GCCTTATGGCCGTCAACATACATATCACCATTATCGAACTCAACGC	255	0.10615932224558189	No Hit
GTCCACGAGGTCTCTAGATGTACCCAATTCTTCGACGTACGCTGCA	248	0.10324514477217377	No Hit
GTCCACGAGGTCTCTATGACGTGTCGATCACTCCTCGTACGCTGCA	243	0.1011635894340251	No Hit
GTCCACGAGGTCTCTGCGTGACAGTTTGTTCAATTCGTACGCTGCA	243	0.1011635894340251	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCTTT	30	4.8514285E-6	40.000004	11
TCTGCCT	185	0.0	40.000004	13
ACAACCT	30	4.8514285E-6	40.000004	19
CTATGTA	60	0.0	40.000004	14
AGCCGGA	30	4.8514285E-6	40.000004	21
TCGCAAA	30	4.8514285E-6	40.000004	28
TGCCCAT	30	4.8514285E-6	40.000004	15
AATTTTT	30	4.8514285E-6	40.000004	26
TGCTTTA	30	4.8514285E-6	40.000004	12
AAGAGTT	30	4.8514285E-6	40.000004	17
CATGCGG	30	4.8514285E-6	40.000004	26
TCACAAT	30	4.8514285E-6	40.000004	23
GCGCGAG	30	4.8514285E-6	40.000004	20
TCCTGCT	30	4.8514285E-6	40.000004	9
TCCTGCA	30	4.8514285E-6	40.000004	15
ATAATTC	30	4.8514285E-6	40.000004	24
ATGGATC	30	4.8514285E-6	40.000004	17
TTTCGAC	30	4.8514285E-6	40.000004	30
CTATAGC	60	0.0	40.000004	14
TCTTATT	120	0.0	40.000004	13

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content